高效液相色谱法_____高效液相色谱法；熊果苷凝胶；熊果苷

高效液相色谱法测定熊果苷凝胶中熊果苷的含量

http://www.100md.com 《广东药学院学报》 2004年第6期

高效液相色谱法,,高效液相色谱法；熊果苷凝胶；熊果苷,1仪器与试药,2方法与结果,3讨论,参考文献

     摘要目的建立测定熊果苷凝胶中熊果苷含量的高效液相色谱方法。方法采用Polaris C18柱，以ψ(甲醇∶水)=20∶80为流动相，检测波长282 nm，苯酚为内标物。结果在5 ~160 μg/mL浓度范围内，熊果苷与内标物的峰面积比与其浓度呈线性关系；方法平均回收率为99.7%，RSD为0.60%。结论本法可用于测定熊果苷凝胶中熊果苷的含量。

    关键词高效液相色谱法；熊果苷凝胶；熊果苷

    Determination of arbutin in arbutin gel by HPLC

    ZHANG Ting, LI Xinzhong, ZHOU Boting

    (Xiangya Hospital, Zhongnan University, Changsha 410008,China)

    Abstract Objective to establish a HPLC method for the determination of arbutin in arbutin gel. Methods the assay was conducted on a PolarisC18 column with methanolwater(20∶80)as mobile phase. The detection wavelength was 282 nm, and phenol was taken as internal standard. Results the linear concentration range of arbutin was 5~160 μg/mL with the recovery of 99.7%(RSD=0.60%). Conclusion the method may be used for the determination of arbutin in arbutin gel.

    Key words HPLC, arbutin gel, arbutin

    熊果苷(对羟基苯βD吡喃葡萄糖苷)是源于绿色植物的天然活性物质 ......

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