高效毛细管电泳法_____头孢他啶；血清；高效毛细管电泳法

高效毛细管电泳法测定血清中头孢他啶的含量

http://www.100md.com 《广东药学院学报》 2004年第1期

高效毛细管电泳法,,头孢他啶；血清；高效毛细管电泳法,1仪器与试药,2方法与结果,3讨论

     摘要采用高效毛细管电泳法测定血清中头孢他啶的含量。毛细管60 cm×75 μm；运行缓冲液25 mmol/L四硼酸钠(pH 82)，高压进样5 s，分离电压20 kV，温度为25 ℃，检测波长为254 nm，地塞米松磷酸钠为内标。头孢他啶在0.625～20 μg/mL浓度范围内线性关系良好(r=0.999 7)，平均回收率为98.81%(n=5，RSD=1.88%)。

    关键词头孢他啶；血清；高效毛细管电泳法

    Determination of ceftazidime in serum by HPCE

    GUO Dan,CHEN Nana, YAN Yuan，MA Yun,HOU Lianbing

    (1.Pharmaceutical Department of Nanfang Hospital,the First Military Medical University,Guangzhou 510515; 2.Institute of Pharmaceutical Sciences,the First Military Medical University,Guangzhou 510515)

    Abstract A HPCE method for the determination of the content of ceftazidime in serum was used. The best condition was achieved with a fused silica capillary tube (60 cm×75 μm), 25 mmol/L sodium tetraborate buffer (pH 8.2), at a constant voltage of 20 kV and temperature at 25 ℃. The UV detection wavelength was at 254 nm and dexamethasone sodium phosphate as internal standard. The calibration curves was linear in the range of 0.625～20 μg/mL for ceftazidime(r=0.999 7)and the average recovery of ceftazidime was 98.81%(n=5 ......

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