基质金属蛋白酶_

在脂多糖和N乙酰半胱氨酸干预体外孵育胎膜中的表达及意义

http://www.100md.com 《现代医学》 2006年第2期

基质金属蛋白酶,,]基质金属蛋白酶；早产；感染；细菌内毒素脂多糖；N乙酰半胱氨酸；逆转录聚合酶链反应,1对象与方法,2结果,3讨论,[

     [摘要]目的探讨感染引发早产的可能机制及N乙酰半胱氨酸(NAC)预防感染引发早产的可行性。方法取20例正常择期剖宫产孕妇(无妊娠合并症及临产征兆)的胎膜在体外进行孵育，共分成5组：0h组(取下后未经孵育的胎膜)，24h组，48h组，72h细菌内毒素脂多糖组(LPS组)，72 h LPS+NAC组(LPS+NAC组)。首先评价体外孵育胎膜的存活力，然后应用逆转录聚合酶链反应(RTPCR)分别测定5组胎膜的基质金属蛋白酶9(MMP9)及金属蛋白酶组织抑制因子1(TIMP1)mRNA表达水平的变化，最后比较LPS组和LPS+NAC组MMP9/TIMP1比值的变化。结果体外孵育胎膜的存活力达到83%±1.9%， RTPCR可见0h组及48h组的MMP9表达微弱，24h组的表达较前两组增强(P<0.05)，LPS组的表达最强(与24h组比较P<0.01)，LPS+NAC组的表达低于LPS组(P<0.01)，各组TIMP1的表达无明显区别(P>0.05)，LPS+NAC组较LPS组MMP9/TIMP1比值明显降低(P<0.01)。结论 MMP9/TIMP1比值升高可能是感染引发早产的机制之一，NAC有望在预防和治疗早产中发挥作用。

    [关键词]基质金属蛋白酶；早产；感染；细菌内毒素脂多糖；N乙酰半胱氨酸；逆转录聚合酶链反应

    Expression and significance of MMP9/TIMP1 in in vitro cultured

    amniochorionic membranes stimulated by

    lipopolysaccharide and NacetylcysteineGAO Yingchun1， JIANG Xiaoqing1，ZHANG Haiping2

    (1. Department of Obstetrics and Gynecology, School of Clinical Medical Science,Southeast University,Nanjing 210009,China;

    2.Department of Obstetrics and Gynecology,the First Hospital of Huaian, Huaian 223000, China)

    Abstract：Objective To discuss the mechanism that infection cause preterm labour, and the ability that Nacetylcysteine(NAC) prevent preterm labour causing by infection. Methods Amniochorionic membranes were collected from 20 pregnancy women undergoing elective cesarean delivery at term, without evidence of active labour and complications of pregnancy,then these amniochorionic membranes were cultured in vitro and were divided into 5 groups: 0h group ......

百拇医药网 http://www.100md.com/html/DirDu/2006/05/31/98/17/14.htm

您现在查看是摘要页，全文长 11506 字符。