陈皮总DNA提取方法的研究
陈皮,,陈皮；总DNA；PVP,1材料与方法,2结果,3讨论,参考文献
摘 要 目的 寻找一种以市售中药材陈皮为材料,可获得高质量的陈皮总DNA的方法。方法 采用CTAB法提取总DNA，在研磨过程加入PVP,经琼脂糖凝胶电泳检测。结果 用非可溶性PVP(Polyvinylpyrrolidone)研磨，CTAB法能较好的去除色素、多糖等干扰物质。 结论 该方法可以用于多糖、色素含量高的中药材的总DNA的提取。关键词 陈皮；总DNA；PVP
Total DNA extraction from Pericarpium Citri Reticulatae
YAN Hanjing, GAO Xiaoxia, CHENG Xiaoying
(1.Departmet of Chinese Materia Medica,Guangdong College of Pharmacy,Guangzhou 510240,China; 2.Departmet of Pharmacy,Guangdong College of Pharmacy,Guangzhou 510240,China)
Abstract Objective To explore the method for extraction of high quality DNA from Pericarpium citri Reticulatae. Methods Two methods, i.e., CTAB, and modified CTAB with PVP, were used to extract total DNA from traditional Chinese medicinal material Pericarpium citri Reticulatae. Results The second method was preferable in removing pigments, polysaccharides, which disturbing PCR. Conclusion This method can be used in extracting DNA from plant Chinese medicinal material that has high contents of pigments and polysaccharides. ......
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