黄芪_____黄芪；血清药理学；大鼠；成骨细胞

黄芪对成骨细胞成骨能力的血清药理学实验

http://www.100md.com 《广东药学院学报》 2004年第5期

黄芪,,黄芪；血清药理学；大鼠；成骨细胞,1材料与方法,2结果,3讨论,参考文献

     摘要目的观察黄芪含药血清对新生大鼠颅骨成骨细胞体系增殖、分化及成骨能力的影响。方法 3月龄SD大鼠12只，随机分3组，分别以生理盐水(NS)、0.5 g/mL及5 g/mL剂量的黄芪灌胃，取大鼠血清经处理终稀释成1∶20、1∶40、1∶80分别加入成骨细胞体系，进行细胞增殖(MTT法)、ALP活性测定以及矿化结节计数。结果黄芪(0.5 g/mL)灌胃的含药血清在1∶80稀释比，MTT法所测吸光度A值及ALP活性显著高于NS对照组；黄芪(5g/mL)灌胃的含药血清在1∶20稀释比，MTT法所测吸光度A值及ALP活性亦显著高于NS对照组；矿化结节计数两组含药血清均显著高于NS对照组。结论黄芪在体内对成骨细胞的成骨作用受药物本身经体内吸收、代谢后的浓度变化调节的影响。

    关键词黄芪；血清药理学；大鼠；成骨细胞

    Effect of serum of Astragalus from rats on the osteogenic capacity of osteoblasts

    WANG Yongdong,WU Xiaorong

    (1. Dongguan University of Technology，Dongguan, Guangdong 523106；2. Guangdong College of Pharmacy，Guangzhou,Guangdong 510240)

    Abstract Objective To ivestigate the effect of serum of Astragalus from SD rats on the osteogenic capacity of calvarium osteoblast in vitro. Methods Twelve SD rats at age of 3 months were randomly divided into 3 groups and treated with saline, Astragalus (05 g/mL and 5 g/mL) respectively for 3 days. The animal serum was prepared for culture in different dilution ratio (1∶20、1∶40、1∶80). MTT, capacity of alkaline phosphatase(ALP), and mineralization were detected to determine osteoblasts osteogenic activities. Results Compared with the control group, both the serum from Astragalus group (0.5 g/mL) at dilution ratio of 1∶80 and the serum from Astragalus group (5 g/mL) at dilution ratio of 1∶20, significantly improved the proliferation and differentiation of osteoblasts; thess two kinds of serum also obviously increased osteoblasts osteogenic capacities. Conclusion Astragalus has twoway regulation capacity on the osteogenic activities of osteoblasts after assimilated and metabolized in vivo. ......

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