肝纤维化_____]_肝纤维化;

丹酚酸B盐对二甲基亚硝胺诱导的肝纤维化大鼠肝脏转化生长因子β1及其受体蛋白表达的影响

http://www.100md.com 《中西医结合学报》 2005年第4期

肝纤维化,,],肝纤维化;,丹酚酸B盐;,二甲基亚硝胺;,TGFβ1;,受体,1材料与方法,2结果,3讨论

     [摘要] 目的：研究中药丹参的有效成分丹酚酸B盐抗肝纤维化作用的机制。方法：Wistar大鼠24只随机分为正常对照组、模型组和丹酚酸B盐治疗组。采用二甲基亚硝胺诱导大鼠肝纤维化模型，丹酚酸B盐治疗组在造模4周后给予丹酚酸B盐治疗4周。治疗结束后，用盐酸水解法检测全部大鼠肝组织中羟脯氨酸的含量，用Western印迹法检测肝组织Ⅰ型胶原蛋白、转化生长因子β1(transforming growth factorbeta1, TGFβ1)、转化生长因子β受体Ⅰ(transforming growth factorbeta receptor type Ⅰ, TβRⅠ)、转化生长因子β受体Ⅱ(transforming growth factorbeta receptor type Ⅱ, TβRⅡ)蛋白的表达。结果：模型组大鼠肝组织中羟脯氨酸的含量、Ⅰ型胶原蛋白、TGFβ1及其受体TβRⅠ和TβRⅡ蛋白的表达较正常对照组均有显著增加，而丹酚酸B盐治疗组的上述指标与模型组比较则均有不同程度的下降。结论：丹酚酸B盐具有明显的抗肝纤维化作用，其作用机制与抑制TGFβ1及其受体蛋白的表达有关。

    [关键词] 肝纤维化; 丹酚酸B盐; 二甲基亚硝胺; TGFβ1; 受体

    Effects of salvianolic acid B on expressions of TGFβ1 and its receptors in liver of rats with dimethylnitrosamineinduced hepatic fibrosis

    WANG XiaoNing, HU YiYang, LIU ChengHai, LIU Ping, ZHU DaYuan

    (Institute of Liver Diseases, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China)

    ABSTRACT Objective: To study the therapeutic mechanism of salvianolic acid B (SAB) in treating hepatic fibrosis. Methods: Twentyfour Wistar rats were randomly divided into 3 groups: normal control group, untreated group and SABtreated group. Rats in the untreated and SABtreated groups were injected intraperitoneally with 0.5% dimethylnitrosamine (DMN) for 4 weeks, daily for 3 days each week at a dose of 10 μg/kg, to induce hepatic fibrosis. Then, rats in the SABtreated group were given SAB orally for another 4 weeks. Hydroxyproline (Hyp) contents in liver tissue of the rats in 3 groups were determined with HCl hydrolysis, and collagen typeⅠ, transforming growth factorbeta 1 (TGFβ1), transforming growth factorbeta receptor typeⅠ(TβRⅠ) and transforming growth factorbeta receptor typeⅡ(TβRⅡ) were detected by Western blotting. Results: The Hyp content and the expressions of collagen typeⅠ, TGFβ1, TβRⅠ and TβRⅡ in the liver tissue of rats in the untreated group increased significantly as compared with those in the normal control group, while those in SABtreated group decreased significantly as compared with those in the untreated group. Conclusion: The therapeutic mechanism of SAB in treating hepatic fibrosis may be related to inhibiting the expressions of TGFβ1 and its receptors. ......

百拇医药网 http://www.100md.com/html/DirDu/2006/05/31/98/19/10.htm

您现在查看是摘要页，全文长 10721 字符。