罗格列酮__(论著)

罗格列酮对鼠血管平滑肌细胞血管紧张素Ⅱ1型受体mRNA表达的影响

http://www.100md.com 《现代医学》 2006年第2期

罗格列酮,,]罗格列酮；鼠血管平滑肌细胞；血管紧张素Ⅱ1型受体mRNA,1材料与方法,2结果,3讨论,[参考文献]

     [摘要]目的探讨罗格列酮对血管紧张素Ⅱ(AngⅡ)诱导鼠血管平滑肌细胞(VSMC)血管紧张素Ⅱ1型受体(AT1R)mRNA表达的影响。方法选6～10代处于对数生长期的鼠血管平滑肌细胞，用终浓度为1μmol·L-1 AngⅡ预先培养2h后分成A、B和C组，分别加入终浓度为20、30、50μmol·L-1罗格列酮培养12h；另外，取预先用终浓度为1μmol·L-1 AngⅡ培养6h的鼠血管平滑肌细胞，加入终浓度为30μmol·L-1的罗格列酮分别再培养0、6、12、24h。提取总RNA，采用逆转录聚合酶链反应(RTPCR)检测平滑肌细胞AT1RmRNA的表达。结果与空白对照组(23.82±4.68)相比，AngⅡ组AT1RmRNA的表达显著升高(P<0.01)；与AngⅡ组(61.04±12.20)比较，罗格列酮干预组(A、B和C组)AT1RmRNA的表达(45.22±7.17、39.82±6.34、34.70±6.69)呈浓度依赖性降低，差异均有显著性(P<0.01)。30μmol·L-1的罗格列酮干预组呈时间依赖性抑制AngⅡ诱导的VSMC AT1RmRNA的高表达(57.37±8.04、46.99±4.81、39.82±6.34、35.71±6.13)，差异均有显著性(P<0.01)。结论一定浓度范围内，罗格列酮可呈浓度和时间依赖性地下调AngⅡ诱导的鼠VSMC AT1RmRNA的表达，这可能是其抗炎、抗动脉粥样硬化(AS)作用的重要机制之一。

    [关键词]罗格列酮；鼠血管平滑肌细胞；血管紧张素Ⅱ1型受体mRNA

    Effect of rosiglitazone on the expression of angiotensin Ⅱ type 1 receptor

    mRNA in mice vascular smooth muscle cellsGUO Xiaozhen, REN Liqun

    (1. Department of Internal Medicine, Jiangyin People′s Hospital, Jiangyin 214400, China;

    2. Department of Internal Medicine, Zhongda Hospital, Southeast University, Nanjing 210009, China) Abstract：Objective To investigate the expression of angiotensin Ⅱ type 1 receptor(AT1R) mRNA in angiotensin Ⅱinduced mice vascular smooth muscle cells(VSMC).Methods VSMC of mice in logarithmic growth phase (passage 610)were incubated with angiotensin Ⅱ(Ang Ⅱ 1μmol·L-1) for 2h,and then were divided into A,B and C groups. 20,30 or 50μmol·L-1 rosiglitazone were coincubated respectively with A,B and C groups for 12h. In addition, VSMC were incubated with Ang Ⅱ (1μmol·L-1) for 6h, and then added 30mol L-1 rosiglitazone and coincubated for 0, 6, 12 or 24h.Total RNA was extracted. AT1R mRNA in VSMC was determined by reverse transcriptionpolymerase chain reaction (RTPCR). Results Compared with the control group(23.82±4.68), the expression of AT1R mRNA of VSMC incubated with Ang Ⅱ was significantly increased(P<0.01). The expression of AT1R mRNA of VSMC was lower in the A or B or C group compared with Ang Ⅱ group(45.22±7.17、39.82±6.34、34.70±6.69 vs 61.04±12.20 ......

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