高效液相法同时测定复方布比卡因乳膏中两组分的含量
乳膏,,乳膏；布比卡因；利多卡因；高效液相色谱法,1仪器与试药,2方法与结果,3讨论,参考文献
摘 要 目的 建立测定复方布比卡因乳膏中利多卡因和布比卡因的含量方法。方法 采用HPLC法同时检测两组分含量。色谱柱：Kromasil C18柱(250 mm×4.6 mm,5 μm)；流动相：甲醇-醋酸盐缓冲液(pH4.6)=65∶35；流速：1.0 mL/min；检测波长：245 nm；进样量：20 μL。结果 空白基质对两组分测定无干扰，在50~300 μg/mL范围内线性关系良好(r=0.9999)。利多卡因和布比卡因的回收率分别为100.1%(RSD=1.28%)和99.0%(RSD=0.84%)。结论 本方法快速简便、准确可靠，可同时测定复方布比卡因乳膏中利多卡因和布比卡因的含量。关键词 乳膏；布比卡因；利多卡因；高效液相色谱法
Simultaneous determination of two components from Compoundbupivacaine ointment by HPLC
DIAO Yuhui,PEI Yunping
(The Affiliated Drum Tower Hospital of Nanjing University， Nanjing 210008, China)
Abstract Objective To establish the method to assay the contents of lidocaine and bupivacaine from compoundbupivacaine ointment. Methods The contents of lidocaine and bupivacaine in compoundbupivacaine ointment were determined by HPLC simultaneously. The analytical column: Kromasil DOS (250 mm×4.6 mm ......
您现在查看是摘要页，全文长 5488 字符。