大黄提取物_____HPLC；大黄提取物；蒽醌

HPLC法测定大黄提取物中5种蒽醌的含量

http://www.100md.com 《广东药学院学报》 2004年第6期

大黄提取物,,HPLC；大黄提取物；蒽醌,1仪器与试药,2方法与结果,3讨论,参考文献

     摘要目的测定大黄溶剂萃取工艺提取物与SFECO2工艺提取物中大黄酚、大黄酸、芦荟大黄素、大黄素、大黄素甲醚等5种蒽醌含量。方法先用CHCl3超声振荡提取游离蒽醌，再将残渣中结合蒽醌进行水解，CHCl3热回流提取；采用HPLC法测定含量。结果与结论从5种蒽醌的转移率来看，SFECO2工艺优于溶剂萃取工艺。

    关键词 HPLC；大黄提取物；蒽醌

    Determination of 5 anthraquinones in rhubarb extracts by HPLC

    ZHENG Zhihua,ZHU Chenchen

    (1.Guangdong Pharmaceutical Association, Guangzhou 510080,China; 2.New Drug Research & Development Center of Guangzhou University, Guangzhou 510405,China)

    Abstract Objective To determine 5 anthraquinones such as chrysophanol, rhein, aloe emodin, emodin and physcion in rhubarb extracts by solvent extraction and SFECO2. Methods CHCl3 was used to extract free anthraquinones by ultrasonic vibration; conjugated anthraquinones in residue was hydrolyzed and then extracted by thermal reflux in CHCl3. The analytical column was Allitima RP C18 (250×4.6 mm, 5 μm). The mobile phase consisted of 0.1%TFAHCN(17∶83). The flow rate was 1.0 mL/min. The UV detection wavelength was 280 nm and the reference wavelength was 600 nm. The column temperature was 25 ℃. Result and conclusion Considering the extracting rate of 5 anthraquinones, SFECO2 was better than solvent extraction. ......

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