8-甲氧补骨脂素__(期刊论文)

8-甲氧补骨脂素脂质体对小鼠黑素瘤细胞黑素生成影响的研究

http://www.100md.com 《广东药学院学报》 2004年第5期

8-甲氧补骨脂素,,8-甲氧补骨脂素；脂质体；黑素；白癜风,1仪器和材料,2方法,3结果,4讨论,参考文献

     摘要目的探讨8-甲氧补骨脂素脂质体(LMOP)对B16F小鼠黑素瘤细胞增殖、黑素含量、酪氨酸酶活性的影响。方法采用体外培养的小鼠B16F黑素瘤细胞株，以等浓度的8-甲氧补骨脂素(MOP)为对照，用MTT法测定细胞增殖情况；NaOH裂解法测定黑素合成；多巴氧化法测定酪氨酸酶活性。结果与等浓度MOP相比，LMOP低浓度(10～50 μmol/L)时显著提高酪氨酸酶活性和黑素含量(P<0.05)，高浓度(100～200 μmol/L)时对细胞的抑制作用更显著(P<0.05)。结论用脂质体作为MOP的载体可以显著提高MOP对靶细胞的作用，为LMOP制剂治疗白癜风的临床应用提供了实验依据。

    关键词 8-甲氧补骨脂素；脂质体；黑素；白癜风

    The Study on regulation of melanogenesis in the responese of murine melanoma cells to 8methoxypsoralen liposomes

    WANG Jun, HAN Ruiling, LUO Sunde, HE Wen

    (Department of Pharmacy，Renmin Hospital of Wuhan University, Wuhan, Hubei 430060)

    Abstract Objective To study the regulation of proliferation, melanogenesis and tyrosinase activity in the response of B16F murine melanoma cells to LMOP. Methods B16F murine melanoma cell was cultured in vitro，and MOP on the same concentration levels was used as control group. MTT was used to study the proliferation of B16 murine cells. The melamin was mesured by NaOHassay, and the tyrosinase activity was detected by DOPAoxidation. Results Compared with MOP groups on the same concentration ......

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