枸杞多糖_____]枸杞多糖;_免疫抑制;

枸杞多糖对荷瘤小鼠肿瘤微环境T淋巴细胞亚群及树突状细胞的影响

http://www.100md.com 《中西医结合学报》 2005年第5期

枸杞多糖,,]枸杞多糖;,免疫抑制;,淋巴细胞亚群;,肿瘤浸润淋巴细胞;,树突状细胞,1材料与方法,2结果,3讨论,[参考文献]

     [摘要]目的：研究枸杞多糖(Lycium barbarum polysaccharide, LBP)对荷瘤小鼠体内肿瘤微环境中T淋巴细胞亚群和树突状细胞(dendritic cells, DCs)的影响，探讨LBP对荷瘤机体免疫逃逸的干预作用。方法：用LBP给H22荷瘤小鼠灌胃，连续2周后，测定肿瘤重量，采用流式细胞术检查肿瘤浸润淋巴细胞(tumorinfiltrating lymphocyte, TIL)中T淋巴细胞亚群和DCs的数量，以及协同刺激分子CD80(B71)表达的变化。结果：LBP可明显抑制肿瘤细胞的生长，增加肿瘤浸润CD4+、CD8+细胞的数量。LBP高剂量组CD4+和CD8+细胞数量的百分比高于模型组(P<0.05)。LBP低剂量和高剂量组肿瘤浸润DCs数量及B71表达均较模型组升高，但差异无统计学意义。结论：LBP的抗肿瘤作用可能与恢复荷瘤小鼠TIL中CD4+、CD8+的细胞数量、解除机体的免疫抑制状态及增强机体的抗肿瘤免疫功能有关。LBP能否恢复荷瘤机体DCs的表型及功能尚待进一步研究。

    [关键词]枸杞多糖; 免疫抑制; 淋巴细胞亚群; 肿瘤浸润淋巴细胞; 树突状细胞

    Effects of Lycium barbarum polysaccharide on tumor microenvironment Tlymphocyte subsets and dendritic cells in H22bearing mice

    HE YanLi, YING Yi, XU YanLi, SU JunFang, LUO Hui, WANG HuiFeng

    (Department of Pathology, Guangzhou University of Traditional Chinese Medicine, Guangzhou, Guangdong Province 510405, China; Department of Hematology, Guangzhou Municipal First People’s Hospital, Guangzhou, Guangdong Province 510180, China)

    ABSTRACT Objective: To study the effects of Lycium barbarum polysaccharide (LBP) on tumor microenvironment Tlymphocyte subsets and dendritic cells in H22bearing mice and the mechanisms for intervention of tumor immune escape by LBP. Methods: H22bearing mice were given LBP orally for two weeks. Tlymphocyte subsets and the phenotypes of dendritic cells in tumorinfiltrating lymphocytes (TIL) were detected by flow cytometry (FCM). Results: LBP could significantly increase the numbers of CD4+ and CD8+ T cells in TIL as compared with those in model control group (P<0.05). In model control group, the number of dendritic cells in tumor microenvironment decreased markedly, while in LBPtreated group, the increased number of dendritic cells and B71 expression were observed, but there were no significant differences between these two groups. Conclusion: LBP has antitumor effect probably by increasing the numbers of CD4+and CD8+ T cells in TIL to relieve the immunosuppression and enhance the antitumor function of the immune system. But whether LBP can recover the phenocyte and function of dendritic cells in H22bearing mice should be further studied. ......

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