益气化瘀方_____]_益气化瘀方;

益气化瘀方及其拆方对大鼠椎间盘纤维环细胞凋亡相关因子的作用

http://www.100md.com 《中西医结合学报》 2005年第6期

益气化瘀方,,],益气化瘀方;,椎间盘;,纤维环细胞;,bcl2;,Bax;,caspase8,1材料与方法,2结果,3讨论,[参

     [摘要] 目的：研究益气化瘀方及其拆方药理血清对大鼠诱导凋亡椎间盘纤维环细胞bcl2、Bax及半胱氨酸蛋白酶8(caspase8)表达的影响。方法：用细胞免疫化学和积分光密度分析等方法比较分析益气化瘀方及其拆方益气方、化瘀方药理血清，以及胰岛素样生长因子1(insulinlike growth factor 1, IGF1)对抗Fas抗体(antiFas antibody)诱导凋亡大鼠椎间盘纤维环细胞bcl2、Bax及caspase8表达的影响。结果：益气化瘀方、益气方、化瘀方及IGF1血清处理组与凋亡模型组相比，bcl2表达升高，Bax及caspase8表达降低，均有统计学意义(P<0.01)；益气化瘀方组与益气方、化瘀方组比较，Bax表达明显降低，有统计学意义(P<0.05)。结论：益气化瘀方及其拆方可以调控大鼠椎间盘纤维环细胞内Bax、bcl2和caspase8表达，其延缓椎间盘退变的机制可能与调控细胞的凋亡相关因子有关。

    [关键词] 益气化瘀方; 椎间盘; 纤维环细胞; bcl2; Bax; caspase8

    Effects of Yiqi Huayu Recipe and its decomposed formulas on apoptosisrelated factors of anulus fibrosus cells in rats

    ZHOU Quan, WANG YongJun, SHI Qi, SUN Peng, ZHOU ChongJian, HU ZhiJun, LIU Mei

    (Spinal Research Institute, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China; College of Acupuncture and Tuina, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China)

    ABSTRACT Objective: To study the effects of Yiqi Huayu Recipe and its decomposed formulasmedicated sera on expressions of bcl2, Bax and caspase8 of apoptotic anulus fibrosus cells in rats. Methods: Immunohistochemical and integral optical density analytic methods were used to observe the effects of Yiqi Huayu Recipe, Yiqi Recipe, Huayu Recipemedicated sera and insulinlike growth factor 1 (IGF1) on the expressions of bcl2, Bax and caspase8 of apoptotic anulus fibrosus cells in rats induced by antiFas antibody. Results: As compared with apoptosis group, bcl2 expression was higher, Bax and caspase8 expressions were lower in Yiqi Huayutreated, Yiqitreated, Huayutreated and IGF1 groups (P<0.01). As compared with Yiqitreated group and Huayutreated group, Bax expression was lower in Yiqi Huayutreated group (P<0.05). Conclusion: Yiqi Huayu Recipe and its decompsed formulas can delay degeneration of the cervical intervertebral disc, which may be due to its action in regulating the expressions of bcl2, Bax and caspase8. ......

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