荧光素酶__(期刊论文)

缺氧调控报告载体的构建与鉴定

http://www.100md.com 《第四军医大学学报》 2006年第7期

荧光素酶,,转录；调控；缺氧;反应元件；荧光素酶；报告基因,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Construction and identification of hypoxia regulatory reporter vectors

    YANG Jie, LI ZhanTing, DU DeWei, CHAI QingHuan, WANG Li, ZHANG HuiZhong, SUN JiFeng

    Department of Nephrology, Central Laboratory, Tangdu Hospital, Fourth Military Medical University, Xian 710038, China

    【Abstract】 AIM: To construct the firefly luciferase reporter gene vectors regulated by hypoxia response promoters. METHODS: The oligonucleotides of hypoxia response element (HRE) and a mutation control were synthesized and cloned into pCIneo vectors to constrct HRE/CMV recombinant vectors. Then HRE/CMV promoters amplified by PCR were subcloned into pGL3Basic vectors. RESULTS: The integrity reporter vectors were verified by restriction enzyme digestion. Also, the sequences of the vectors were detected and aligned to the expected sequences. CONCLUSION: Luciferase reporter vectors regulated by HRE/CMV promoters were successfully constructed, which makes it possible to further investigate their potency of hypoxia regulation. ......

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