肝细胞_____]衰老大鼠;_肝细胞;_p16;

益气活血方药对老年大鼠肝脏衰老相关基因表达的影响

http://www.100md.com 《中西医结合学报》 2005年第5期

肝细胞,,]衰老大鼠;,肝细胞;,p16;,p21;,增殖细胞核抗原;,cyclin,D1;,cyclin,E;,益气方;,活血方,1材料与方法

     [摘要]目的：观察益气、活血方药对老年大鼠肝脏衰老相关基因表达的影响。方法：以自然衰老大鼠为衰老模型，采用RTPCR、Western blotting法研究益气、活血方药对老年大鼠肝脏p16、p21、增殖细胞核抗原(proliferating cell nuclear antigen, PCNA)、cyclin D1和cyclin E mRNA转录和蛋白表达的影响。结果：老年大鼠肝脏p16、p21、cyclin D1 mRNA转录和蛋白的表达增加；PCNA、cyclin E mRNA转录和蛋白的表达降低。与老年大鼠比较，益气、活血方药可下调老年大鼠肝脏cyclin D1 mRNA转录和蛋白表达，上调促进增殖基因cyclin E mRNA转录和蛋白表达，但对p16、p21和PCNA的调节作用不明显。结论：益气、活血方药主要通过调控cyclin D1和cyclin E mRNA转录和蛋白表达而促进细胞增殖。

    [关键词]衰老大鼠; 肝细胞; p16; p21; 增殖细胞核抗原; cyclin D1; cyclin E; 益气方; 活血方

    Effects of recipes for replenishing qi and activating blood on senescence related gene expressions in the liver of aging rats

    LI ZhiPing, ZHAO WeiKang, XU PinChu, CHEN FangMin, JIN GuoQin, YAO MingZhong

    (Department of Biochemistry, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; Institute of Geriatrics, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China)

    ABSTRACT Objective: To observe the effects of recipes for replenishing qi and activating blood on p16, p21, proliferating cell nuclear antigen (PCNA), cyclin D1 and cyclin E gene expressions in the liver of aging rats. Methods: A recipe for replenishing qi and a recipe for activating blood were administered to aging rats respectively, and the effects of the above recipes on the expressions of senescence related genes (p16, p21, PCNA, cyclin D1 and cyclin E) were examined by RTPCR and Western blotting methods. Results: The expressions of p16, p21 and cyclin D1 mRNAs and proteins in the liver of the untreated aging rats were upregulated, while the expressions of PCNA and cyclin E mRNAs and proteins decreased. As compared with the untreated aging rats, both recipes could downregulate the expressions of cyclin D1 mRNA and protein and upregulate the expressions of cyclin E mRNA and protein, but had no obvious effects on the expressions of mRNAs and proteins of p16, p21 and PCNA. Conclusion: Recipes for replenishing qi and activating blood can improve the liver cell proliferation of aging rats via downregulating the expressions of cyclin D1 mRNA and protein and upregulating the expressions of cyclin E mRNA and protein. ......

百拇医药网 http://www.100md.com/html/DirDu/2006/05/31/98/24/34.htm

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