Effect of ischemic preconditioning on energy metabolism and expressions of Bclª²2 and Bax in ischemia/reperfusion of rat small intestine

    LIU Xin, LIU Xin

    Department of Anesthesiology, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China

    ¡¾Abstract¡¿ AIM: To study the effect of ischemic preconditioning on cell apoptosis following acute intestinal ischemia/reperfusion in rats and explore their relationship. METHODS£ºThirtyª²six SD rats were randomized into 3 groups each consisting of 12 rats: shamª²operated control group(C group), ischemic reperfusion group(I/R group, the anterior mesenteric artery occlusion for 60 min followed by reperfusion for 120 min ), ischemic preconditioning group (IPCª²I/R, clamping the anterior mesenteric artery for 10 min followed by unclamping it for 10 min, all for 4 times before I/R). Immunohistochemistry was used to detect the expressions of Bclª²2 and Bax. Twentyª²four fields of vision were selected in each group to determine A value. TUNEL method was applied to detect apoptotic intestinal cells, and to calculate the apoptotic index. RESULTS£ºThe contents of ATP in C and IPCª²I/R groups were significantly higher than that in I/R group (P<0.05)£»the A value of Bclª²2 in I/R group was remarkably lower than that in C group (P<0.05), and the A value of Bclª²2 in IPCª²I/R group was obviously higher than those in C and I/R groups (P<0.01); the A value of Bax in I/R group was significantly higher than those in C and IPCª²I/R groups(P<0.01), and the A value of Bax in IPCª²I/R group was remarkably higher than that in C group (P<0.05). Compared with that in C group, the apoptotic indexes in I/R and IPCª²I/R group were significantly higher (P<0.01 and P<0.05); Compared with that in I/R group, the apoptotic index in IPCª²I/R group was significantly lower (P<0.01). CONCLUSION£ºIschemic preconditioning can curb ATP degeneration,enhance the expression of Bclª²2, decrease the expression of Bax, inhibit the apoptosis following small intestinal ischemic reperfusion. ......