灯盏乙素_____灯盏乙素；高效液相色谱法；灯盏细辛

HPLC法测定不同产地灯盏细辛中灯盏乙素的含量

http://www.100md.com 《第四军医大学学报》 2006年第9期

灯盏乙素,,灯盏乙素；高效液相色谱法；灯盏细辛,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Determination of scutellarin in Erigeron breviscapus from different places by HPLC

    LIU Peng, ZHOU Jun, QIN XiangYang, WANG QiaoFeng, LI XiaoYe

    Department of Chemistry, School of Pharmacy, Fourth Military Medical University，Xian 710033，China

    【Abstract】 AIM: To develop a HPLC method to determinate the proportion of scutellarin in Erigeron breviscapus from different places of production. METHODS: The analytical column was a ZODBAXC18 (4.6 mm×150 mm) filled with a 5 μm stationary phase. The mobile phase consisted of methanol(0.02 mol/L) phosphate buffer solution [pH 5.2 (35∶65)]. The flowrate was 0.8 mL/min. The injection volume was 20 μL. The Detector was set at 335 nm. The column temperature was 25℃. RESULTS: The calibration curve was linear over the range of 0.74-3.70 μg (r=0.999 6). The average recovery of the method was 97.24%, RSD 0.82% (n=6). CONCLUSION: The proposed method would be used in quantitative analysis of scutellarin in Erigeron breviscapus. The method is convenient, economical and accurate with good reproducibility. ......

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