Establishment and improvement of rat orthotopic liver transplantation model with twoª²cuff technique

    LI Jun, DOU Keª²Feng, ZHAO Qingª²Chuan, ZHANG Hongª²Tao, KONG Yaª²Lin, ZHANG Fuª²Qin

    Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, Xi¬ðan 710033, China

    ¡¾Abstract¡¿ AIM: To improve the model of rat orthotopic liver transplantation and study the rates of success and longª²term survival of the model. METHODS: On the basis of twoª²cuff technique of Kamada, we¬ðve improved the techniques of separating, perfusing donor liver, the shearing and anastomosis of the superior and inferior caval veins, the bile duct anastomosis, and so on. RESULTS: Operation had been performed 80 times with improved techniques and 92 times with conventional techniques. In contrast to conventional techniques, the improved techniques reduced the average time of donor operation and that of recipient¬ðs to (24.3¡À3.2) min and (56.4¡À5.0) min(P<0.05), elevated 48ª²hour survival rate to 91.3%(73/80) and 1ª²week survival rate to 87.5%(70/80)(P<0.05). CONCLUSION: The improved techniques have raised the quality of donor liver and the success rate of the model. ......