抗HBsAg-Fab重组体在小鼠肝脏的表达
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唐 莉, 曾志贵, 潘 桃, 袁 劲, 陈必成,
真核表达;小分子抗体;乙肝表面抗原;小鼠唐莉,曾志贵,潘桃,袁劲,陈必成,陈忠华,陈知水.抗HBsAg-Fab重组体在小鼠肝脏的表达. 世界华人消化杂志2006;14(16)1554-15
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唐莉, 曾志贵, 潘桃, 袁劲, 陈忠华, 陈知水, 华中科技大学同济医学院附属同济医院器官移植研究所 国家教育部/卫生部重点实验室 湖北省武汉市 430030
陈必成, 温州医学院附属第一医院移植中心 浙江省温州市 325000
唐莉, 2001年皖南医学院本科毕业, 2006年华中科技大学同济医学院博士毕业, 主要从事移植免疫及抗体工程研究.
国家自然科学基金面上项目资助, No. 30471695
通讯作者: 陈知水, 430030, 湖北省武汉市汉口解放大道1095号, 同济医院器官移植研究所. zschen@tjh.tjmh.edu.cn
电话: 027-83662592
收稿日期: 2006-03-24 接受日期: 2006-04-13
Expression of recombination human anti-HBsAg Fab in murine liver
Li Tang, Zhi-Gui Zeng, Tao Pan, Jin Yuan, Bi-Cheng Chen, Zhong-Hua Chen, Zhi-Shui Chen
Li Tang, Zhi-Gui Zeng, Tao Pan, Jin Yuan, Zhong-Hua Chen, Zhi-Shui Chen, Institute of Organ Transplantation; Key Laboratory of Organ Transplantation, Ministry of Education/Ministry of Health, Tongji Hospital of Tongji Medical College, Huazhong Univerisity of Science and Techology, Wuhan 430030, Hubei Province, China
Bi-Cheng Chen, Center of Transplantation, the First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, Zhejiang Province, China
Supported by National Natural Science Foundation of China, No. 30471695
Correspondence to: Dr. Zhi-Shui Chen, Institute of Organ Transplantation, Tongji Hospital of Tongji Medical College, Huazhong Univerisity of Science and Techology, 1095 Jiefang Street, Wuhan 430030, Hubei Province,China. zschen@tjh.tjmh.edu.cn
Received: 2006-03-24 Accepted: 2006-04-13
Abstract
AIM: To evaluate the expression of recombinant and soluble human anti-HBsAg Fab in murine liver.
METHODS: The 5-40 μg recombinant plasmid pXXUF1-HBs-Fab was transfected by hydrodynamics-based administration via tail vein into C57BL/6 mice within 5 s. Meanwhile the empty plasmid transfection group and non-transfection group were established. The serum and tissue samples from liver, kidney and spleen of these mice were collected 3 d after transfection. The expression of genetically engineered Fab antibody in vivo was identified by reverse transcription-polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry both at mRNA and protein level ......
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