胆结石_____]_胆结石;_升清胶囊;

升清胶囊对豚鼠胆石病相关基因的影响

http://www.100md.com 《中西医结合学报》 2005年第3期

胆结石,,],胆结石;,升清胶囊;,BUGT;,胆固醇7α羟化酶;,基因,1材料与方法,2结果,3讨论,[参考文献]

     [摘要] 目的：探讨中药升清胶囊在分子层面防治胆石病的机制。方法：雌性豚鼠60只，随机分成正常组(喂正常饮食作为对照)、模型组(喂低蛋白饮食致石)和中药组(喂低蛋白饮食致石加中药升清胶囊治疗)。6周后处死并取材，观察动物成石情况，检测肝组织胆红素尿苷二磷酸葡萄糖醛酸基转移酶(bilirubin UDPglucuronosyltransferase, BUGT)mRNA及胆固醇7α羟化酶(cholesterol 7αhydroxylase, CYP7A1)mRNA表达。结果：各组豚鼠成石比例分别为正常组2/14、模型组为9/11、中药组为4/14，组间有显著差异(P<0.05)，正常组和中药组豚鼠的肝组织BUGT mRNA及CYP7A1 mRNA表达明显高于模型组(P<0.05)。结论：中药升清胶囊可能是通过上调肝组织中BUGT mRNA和CYP7A1 mRNA的表达，干预胆红素和胆固醇代谢，抑制致石性胆汁形成，起到预防胆结石的作用。

    [关键词] 胆结石; 升清胶囊; BUGT; 胆固醇7α羟化酶; 基因

    Effects of Shengqing Capsules on cholelithiasisrelated genes in guinea pigs

    ZHU PeiTing, ZHANG JingZhe, ZHANG XueLin, YUAN ZuoBiao, ZHANG HongYing, GAO Ju

    (Institute of Chinese Traditional Surgery, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China)

    ABSTRACT Objective: To explore the molecular mechanisms of Shengqing Capsules in treating cholelithiasis. Methods: Sixty female guinea pigs were randomized into 3 groups: group Ⅰ (fed with normal diet), group Ⅱ (fed with lowprotein diet) and group Ⅲ (fed with lowprotein diet and Shengqing Capsules). After sixweek feeding, the gallstone formation and the expressions of bilirubin UDPglucuronosyltransferase (BUGT) mRNA and cholesterol 7αhydroxylase (CYP7A1) mRNA were observed. Results: The proportions of stoneformed in groups Ⅰ, Ⅱ and Ⅲ were 2/14, 9/12 and 4/14, respectively. There were significant differences among the three groups (P<0.05). The expressions of BUGT and CYP7A1 mRNAs were higher in both group Ⅰ and group Ⅲ as compared with those in the group Ⅱ (P<0.05). Conclusion: Shengqing Capsules can reduce the rate of stoneformation, which may be due to its interference of metabolism of bilirubin and cholesterol and upregulation of the expressions of BUGT and CYP7A1 mRNAs. ......

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