Detection of transcription mediator CBF1 expression in mice bone tissue

    WANG Shengª²Chao1, Kawashima Nobuyuki2, Sakamoto Kei3, Katsube Kenª²Ichi3, Shindo Kentaro2, Takagi Minoru3, Suda Hideaki2, SHI Junª²Nan1

    1Department of Endodontics, Stomatological College, Fourth Military Medical University, Xi¬ðan 710033, China,2Pulp Biology & Endodontics, 3Molecular Pathology, Graduate School of Tokyo Medical & Dental University,Tokyo 113ª²8549, Japan

    ¡¾Abstract¡¿AIM: To investigate the expression pattern of transcription mediator CBF1 in adult mice bone tissue and in embryonic bone-forming cells. METHODS: Real time RTª²PCR was used to determine the mRNA level of CBF1 in multiple organs of mice, including bone tissue. In situ hybridization was used to detect the expression of CBF1 during osteogenesis in mice embryos. RESULTS: Expression of CBF1 is ubiquitous in adult mice organs. Bone tissue exhibits relatively high level of CBF1 expression (although not the highest). Strong staining was found in embryonic phalange and rib chondrocytes of mice. Osteoblasts in rib and vertebra exhibited extremely strong staining. Faint staining was also observed in osteocytes. CONCLUSION: Transcription mediator CBF1 is involved both in osteogenesis and in the remodeling and metabolism of mature bone. ......