成骨细胞_____细胞培养；_成骨细胞；

应用活性玻璃/rhBMP2构建生物活性材料支架的相容性研究

http://www.100md.com 《中国矫形外科杂志》 2006年第6期

成骨细胞,,细胞培养；,成骨细胞；,生物活性玻璃；,重组人骨形态发生蛋白2；,生物相容性,1料和方法,2结果,3讨论,参考文献：

     观察BG/rhBMP2构建的三维立体材料支架的细胞相容性、组织相容性，为进一步改善材料性能提供依据。[方法]体外培养的兔成骨细胞分别与BG和BG/rhBMP2材料支架联合培养，倒置显微镜、扫描电镜观察细胞黏附情况，MTT法分析细胞增殖情况；将复合培养的两种支架材料植入兔肌袋中，同期观察复合材料及空白对照组，分别于术后2、4、8、12周来评价其组织相容性。[结果]MTT法结果显示：随着培养时间的延长，各组细胞数量都明显增加，各时间点复合材料支架组细胞数高于空白对照组，差异有显著性意义，复合材料支架BG/rhBMP2在细胞贴壁时间和细胞活性方面均较单纯支架优良；两种支架材料在体内均无明显炎症反应。[结论]BG/rhBMP2是一种具有良好生物相容性的支架材料，有望在骨组织工程中得到广泛应用。

    关键词：细胞培养；成骨细胞；生物活性玻璃；重组人骨形态发生蛋白2；生物相容性

    Compatibility research of threedimensional bioactive glass/rhBMP2 materialscaffolds∥DING Jinyong, JIN Anmin, MIN Shaoxiong,et al.Department of Orthopaedics Surgery, Zhujiang Hospital, Guangzhou 510280

    Abstract：[Objective]To investigate the compatibility of tissue and cell in the threedimensional porous bioactive glass/recombine human Bone Morphogenetic Protein2 (BG /rhBMP2)materialscaffolds and provide a reference for improving the constitution of composite material.[Method]The material of BG and BG/rhBMP2 were cultured in vitro with rabbit osteoblasts respectively. The morphology and adhesion were examined by inverted microscope and scanning electron microscope, and the cell proliferation was detected by MTT assay. The composite materialscaffold were embeded in rabbit's muscle and incisions were observed. The specimens were harvested in 2, 4, 8 and 12 weeks respectively and examined by histology to evaluate the tissue response.[Result]The results showed the cells on BG and BG /rhBMP2 were good adhension and activity, the two materialscaffolds had no apparent inflammatory reaction in vivo. MTT assay showed the number of the cells in each group were significantly increased along with the culture time prolonged. The number of cells in the BG and rhBMP2 group was higher than that of the control group at different intervals, with a significant difference. The number of cells in the BG/rhBMP2 was not evidently higher than that of the BG with a significant difference.[Conclusion]The porous composite materialscaffold of BG/rhBMP2 has good biocompatibility with the hope to wide use in bone tissue engineering. ......

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