Cartilage induction by bone morphogenetic protein in tracheal graft at different intervals

    LIU Yong,LI Xiaoª²Fei,YAN Xiaoª²Long,LU Qiang,WANG Lu,WANG Jian

    Department of Thoracic Surgery , Tangdu Hospital , Fourth Military Medical University , Xi¬ðan 710038, China

    ¡¾Abstract¡¿ AIM: To investigate the different effects of recombinant human bone morphogenetic proteinª²2 in autograft to induce new cartilage formation at different intervals. METHODS: Thirtyª²six mongrel dogs were randomly divided into group A and B, five ring cervical tracheal segment was harvested as the graft and transplanted into the omentum. rhBMPª²2 with collagen as a carrier was injected into the soft tissue between cartilaginous rings in Group A. All the animals were sacrificed in the 1, 2, 3, 5 ,9 and 12 postoperative week respectively, the postmortem specimens were examined histologically and quantitive measurement of ostcocalcium(BGP) level. RESULTS: Chondroregeneration occured in rhBMPª²2 injected area at all intervals, more new cartilage was induced from 2 to 5 postoperative week. BGP level increased with the extention of time in every group, except 1 postoperative week(P>0ª±05), group A were significantly higher than group B at other intervals(P<0ª±05). CONCLUSION: The rhBMPª²2 could induce new cartilage formation in the tracheal graft, but its effects were different at different intervals. ......