Serodetection of hepatitis B virus tranª²scripts and its significance in verticaltransmission

    CUI Yun1,ZHANG Wei1, SU Qin1, ZHU Shaoª²Jun1, FENG Yingª²Ming2£¬Wu Tao3, WANG Qingª²Tu4,GUO Yong4

    1Department of Pathology,2Department of Oncology,3Department of General Surgery, Tangdu Hospital, Fourth Military Medical University, Xi¬ðan 710038, China, 4Women and Children Health Center, Weifang 261000 , China

    ¡¾Abstract¡¿ AIM: To investigate the significance of viral transcripts in HBV vertical transmission through serodetection.METHODS: Viral nucleic acids were extracted from sera of the HBV surface antigen positive mothers and of their newborn infants. HBV DNA and RNAs, including the fullª²length and truncated RNA molecules, were amplified respectively through PCR and RTª²PCR targeted to the HBV X proteinª²encoding region. RESULTS: The viral DNA, fRNA and trRNA were detected in HBeAg(+)/ HBeAg(-)mothers at frequencies of 75%, 65%, 70% and 37ª±5%, 20ª±8%, 58ª±3%, respectively. The differences between these two groups were significant for the former two parameters. HBsAg£¬viral DNA and fRNA were detected in much less infant samples, respectively at frequencies of 9ª±1% ......