Effects of different concentrations of glucose on expression of TRAIL in MG63 cells

    ZHOU Wei, JI Qiuª²He, ZHANG Nanª²Yan, HUANG Weiª²Quan

    Department of Endocrinology, Xijing Hospital, Department of Histology and Embryology, School of Basic Medicine, Fourth Military Medical University, Xi¬ðan 710033, China

    ¡¾Abstract¡¿ AIM: To observe the regulative effects of different concentrations of glucose on the expressions of tumor necrosis factorª²related apoptosisª²inducing ligand(TRAIL),osteoprotegerin(OPG),and the ligand of osteoprotegerin(OPGL) in osteosarcoma MG63 cells,and to study the role of nonª²physiological concentration of glucose in pathogenesis of diabetic osteoporosis. METHODS: MG63 cells were incubated with glucose at the concentration of 5.5, 16.7, 33.3 mmol/L for 24 h respectively.The expressions of TRAIL, OPG and OPGL mRNA were examined by reverse transcriptase(RT)ª²PCR.Expression and distribution of TRAIL in MG63 cells were investigated by immunohistochemical method. RESULTS: The mRNA expressions of TRAIL and OPGL in MG63 cells increased in the order of control group, 5.5, 16.7, 33.3 mmol/L glucose groups(P<0.05),and the expression of OPG mRNA decreased in the same order(P<0.05).TRAIL mRNA level in 33.3 mmol/L group was significantly higher than that in control group[(1.004¡À0.070) vs (0.740¡À0.023), P<0.05], and the intensity of immunostaining for TRAIL was stronger than that in control group. CONCLUSION: High concentration of glucose could lead to the increasing expression of some boneª²resorbing cytokines such as TRAIL and OPGL in osteoblasts but the decreasing expression of OPG, which may be one of the key pathogenetic factors of diabetic osteoporosis. ......