【摘要】 目的 研究佛波酯 (12-O-tetradecanoylphorbol-13-acetate，TPA) 对人食管癌细胞的影响。方法 细胞增殖试验采用MTT法和细胞计数的方法，细胞核形态的测量采用计算机辅助的图像分析系统，分别测量计算细胞核面积(NA)、核周长(NP)、最大直径(Dmax)、 最小直径(Dmin)、核浆比(NPA)、核轴比(NAR)、核形状因子(NRF)等。结果 TPA没有抑制人食管癌Eca109细胞增殖和改变细胞核形态。结论 TPA对人食管癌Eca109细胞可能没有促分化作用。

     【关键词】 佛波酯； 食管癌细胞； 细胞核形态

    Effects of TPA on proliferation and nuclear morphometry of human esophageal cancer Eca109 cells

    GUO Yu-zhong,JIA Lin-jing,GAO Na,et al.Laboratory for Cell Biology，Medical College，Zhengzhou University,Zhengzhou 450052,China

    【Abstract】 Objectives To investigate the effects of 12-O-tetradecanoylphorbol-13-acetate (TPA) on human esophageal cancer Eca109 cells. Methods The sensitivity of TPA to Eca109 cells was determined with MTT assay and counting cell numbers. After the cells were stained with HE，the measurements of nuclear morphometry were performed with computer-assisted image analysis system. Results TPA did not inhibit the cells proliferation and decrease the nuclear size. Conclusions TPA may not induce Eca109 cells to differentiate.

    【Key words】 12-O-tetradecanoylphorbol-13-acetate (TPA); esophageal cancer cell line; nuclear morphometry ......