宫颈癌_____宫颈癌；Skn1a；克隆；表达

宫颈癌组织中转录因子Skn1a cDNA的克隆及表达

http://www.100md.com 《新疆医科大学学报》 2006年第5期

宫颈癌,,宫颈癌；Skn1a；克隆；表达,宫颈癌组织中转录因子Skn1acDNA的克隆及表达,摘要,关键词,1材料与方法,2结果,3讨论,参考文献

     摘要: 目的：从宫颈癌组织中扩增与上皮细胞分化和人乳头瘤病毒(HPV)基因表达相关的转录因子Skn1a cDNA，进而在真核细胞中表达该转录因子以了解其功能。方法：从宫颈癌组织中提取总RNA，以此为模板，RTPCR扩增Skn1a cDNA片段，将该cDNA 片段克隆至pMD18T载体并进行序列分析，构建Skn1a真核表达载体，并转染Hela细胞，检测其表达情况。结果： RTPCR扩增得到约1 300 bp的cDNA 片段，将其克隆至pMD18T载体，DNA测序表明，得到片段长度为1 308 bp ,为转录因子Skn1a基因编码的cDNA，获得的Skn1a cDNA序列与已公布的Skn1a cDNA序列具有99.85%同源性, 有1个碱基的差异，但并未导致编码氨基酸的改变，继而构建了Skn1a真核表达载体pcDNA3/Skn1a，其转染Hela细胞后可正确表达。结论：转录因子Skn1a在宫颈癌组织中有一定量的表达，并在体外培养的Hela细胞中获得表达。

    关键词：宫颈癌；Skn1a；克隆；表达

    cDNA cloning and expressing of human Skn1a cDNA from

    cervical carcinoma tissue

    WANG Yanping, MA Zhenghai, YU Meng, et al

    (Key Laboratory of Molecular Biology, College of Life Science and Technology, Xinjiang University,

    Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, Urumqi 830046, China)

    Abstract: Objective: To clone cDNA of human transcription factor Skn1a (related to epidermal differentiation and HPV gene expression) gene from cervical carcinoma tissue and construct the eukaryotic expression vector of the Skn1a cDNA. Methods: The Skn1a cDNA fragment was amplified by RTPCR from total RNA that was extracted from cervical carcinoma tissue ......

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