用原子力显微镜表征DNA纯化效果的实验研究
原子力显微镜,,]原子力显微镜;表征;脱氧核糖核酸;纯化,[摘要],[关键词],1材料和方法,2结果,3讨论,[参考文献]
[摘要]目的：运用原子力显微镜(AFM)表征3种DNA纯化试剂盒对DNA的纯化效果。方法：PCR扩增K562/A02细胞mdr1基因DNA,分别以3种不同的DNA纯化试剂盒对PCR产物进行纯化后，按终浓度为10 ng·μl-1固定在用1 ng·μl-1 L型多聚赖氨酸预先处理过的新剥离的云母片上，用AFM获取DNA扫描图像，分析评价3种DNA试剂盒对DNA的纯化效果。结果:用AFM成功表征3种纯化试剂盒纯化后DNA片段，获得了清晰的DNA图像。对3种DNA纯化试剂盒的纯化效果作出评价，建议其中的两种DNA纯化试剂盒的DNA纯化产物可用于AFM的DNA 分析。结论：用AFM表征DNA时对DNA的纯度要求较高。通过对3种DNA纯化试剂盒纯化效果的比较，为AFM观测DNA样本的纯化方法提供了较好的方案。
[关键词]原子力显微镜;表征;脱氧核糖核酸;纯化
[中图分类号]Q336
Identification of DNA purification effect by atomic force microscopy
XIONG Jie1，CHEN Baoan1，BA Long2，GAO Feng1，GAO Chong1，DING Jiahua1，SUN Yunyu1,CHENG Jian1，WANG Jun1, ZHAO Gang1, DU Juan1, LU Zuhong 2
(1.Department of Hematology, Zhongda Hospital, Southeast University, Nanjing 210009, China;
2.Electronic Lab of Molecule and Biomolecule, Southeast University, Nanjing 210096, China)
Abstract：Objective To identificate purification effect of three kinds of DNA purification kits by atomic force microscopy(AFM).Methods Cultivateion and PCR technique was used to amplify K562/A02 cells with DNA of mdr1gene. The amplified products were purified by three kinds of DNA purification kits ......
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