肝移植_____肝移植；_大鼠；

大鼠原位肝移植模型的手术操作技巧探讨

http://www.100md.com 《新疆医科大学学报》 2006年第5期

肝移植,,肝移植；,大鼠；,动物模型,摘要,关键词,1材料与方法,2结果,3讨论,参考文献

     摘要：目的：探讨建立稳定大鼠原位肝移植模型的手术操作技巧。方法：采用二袖套法行大鼠原位肝移植，供体经腹主动脉进行肝脏冷灌注，肝上下腔静脉采用手缝合法进行吻合；门静脉和肝下下腔静脉用袖套法吻合；胆总管采用单管内支架胆管端端吻合。结果：大鼠原位肝移植52例，手术成功45例(86.5%)，1周内存活43例(82.7%), 1个月内存活36例(69.2%)，供体手术时间(30.6±4.0)min,受体手术时间(46.0±5.5)min,无肝期为(20.0±2.5)min。结论：大鼠原位肝移植模型稳定可靠，可为肝移植提供理想的研究手段；良好的肝脏灌注，改进的肝上下腔静脉吻合法可以缩短无肝期，提高大鼠肝移植的生存率。

    关键词：肝移植；大鼠；动物模型

    Technique of orthotopic liver transplantation in ratsPaizula Shalayiadang, WEN Hao

    (Department of General Surgery, First Affiliated Hospital ,Xinjiang Medical University,

    Urumqi 830054, China) Abstract: Objective: To investigate the surgical technique of establishing a reliable rat model of orthotopic liver transplantation. Methods: The model was established with two cuff technique; The donor was perfused through the abdominal aorta with cold perfusate; The anastomosis of the suprahepatic vena cava was sutured endto end with 8~0 nylon line, and the continuity of portal vein and infrahepatic vena cava were established by means of cuff method respectively. The bile duct anastomosis was performed with internal stent. Results: Fifty two cases of rat orthotopic liver transplantation were performed and the operative successful rate was 86.5% (45/52), 1 week and 1 month survival rates were 82.7% (43/52), and 69.2%(36/52) respectively ,the resipient underwent an anhepatic period of (20.0±2.5)min,the operative time of donor and recipient were(30.6±4.0)min and(46.0±5.5)min respectively. Conclusion: This model could be used as an effective measure for the study of liver transplantation in rats; The meticulous surgical performance and shortening the anhepatic phase of recipient are the keys to successful operation. ......

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