水飞蓟素脂质体对小鼠CCl4急性肝损伤保护作用的实验研究
水飞蓟素脂质体,,水飞蓟素脂质体;,益肝灵片;,肝损伤,摘要,关键词,1材料与方法,2结果,3讨论,参考文献
摘要: 目的:研究水飞蓟素脂质体(LSIL)对四氯化碳(CCl4)所致小鼠急性肝损伤的保护作用。方法:昆明种小鼠80只,随机分为空白组、CCl4急性肝损伤模型组及LSIL低、中、高剂量组和益肝灵(TSIL)低、中、高剂量组共8组,测量并比较各组小鼠血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)水平及肝组织匀浆中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSHPX)水平和丙二醛(MDA)含量,并观察各组小鼠肝组织病理学改变。结果:与CCl4急性肝损伤模型组相比,LSIL各剂量组血清ALT、AST水平明显降低(P<0.05~0.01),LSIL各剂量肝组织匀浆GSHPX水平及LSIL高剂量组SOD水平明显升高(P<0.05~0.01),并具有剂量依赖性,LSIL高剂量组肝组织匀浆MDA含量明显降低(P<0.01)。LSIL对ALT、SOD、GSHPX水平的作用优于相应剂量TSIL组(P<0.05~0.01)。组织学观察LSIL中、大剂量组的肝损伤明显轻于模型组和相应剂量TSIL组。结论:LSIL对小鼠CCl4肝损伤有明显的保护作用,且其保护作用明显优于TSIL。关键词: 水飞蓟素脂质体; 益肝灵片; 肝损伤
The protective effect of silymarin liposomes against
experimental liver injury in mice
XU Hongxia, GAO Xiaoli
(Department of Pharmacy, College of Pharmacy, Xinjiang Medical University, Urumqi 830054, China)
Abstract: Objective: To investigate the protective effect of silymarin liposomes(LSIL) on the CCl4induced acute liver injury in mice. Methods: Kun ming mice were randomly divided into normal control group, model control group, high, middle and low dose groups of LSIL and high, middle and low dose groups of TSIL. The model of hepatic damage were induced by CCl4. The Alanine aminotransferase(ALT), Aspartate aminotransferase(AST) levels in serum , malondialdehyde(MDA) content, superoxide dismutase(SOD), glutathione peroxidase(GSHPX) levels in liver homogenate were examined and dimensions of hepatic damage in the histopathology were observed. Results: LSIL could inhibit the rising of serum ALT, AST levels caused by CCl4 in doseresponse manner(P<0.05~0.01). Meanwhile, LSIL also decreased MDA content(P<0.01) and prevented the reduction of SOD, GSHPX levels in the liver homogenate(P<0.05~0.01). The effects of LSIL on the ALT level in serum and SOD, GSHPX levels in liver homogenate against experimental liver injury in mice were more powerful than that of TSIL of the same dose(P<0.05~0.01). The liver pathological observation showed that the liver injury of LSIL (150,300 mg/kg.d) were significantly less than that of the model group and TSIL of the same dose. Conclusion: The protective effect of LSIL on the CCl4induced acute liver injury in mice was more potent than that of TSIL in the same dose. ......
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