组织工程化骨_____组织工程化骨；_骨缺损；

间充质干细胞体内成骨的实验研究

http://www.100md.com 《中国矫形外科杂志》 2006年第9期

组织工程化骨,,组织工程化骨；,骨缺损；,新生骨；,间充质干细胞,摘要,关键词,1材料与方法,2结果,3讨论,参考文献

     摘要：[目的]探讨间充质干细胞在体内的成骨能力。[方法]从羊髂嵴处抽取10~15 ml骨髓组织，用Percoll分离液按梯度离心法分离出间充质干细胞，培养、增殖后种植在多孔β磷酸三钙上，构建组织工程化骨，植入8只羊的左后肢跖骨缺损区(长21 mm)作为实验组；单纯植入多孔β磷酸三钙陶瓷材料的8只羊作为对照组；分别在术后6、12、24周处死动物行放射学、组织学和生物力学检测。[结果]放射学和组织学检测，术后6周实验组即可见有新骨生成，对照组则无明显新骨生成；骨缺损部位新生骨样组织、编织骨和板状骨出现的时间实验组也都较对照组早，并且不经软骨介导即能直接成骨，而对照组从两端以“爬行替代”方式成骨。术后24周，放射学和生物力学检测显示实验组骨缺损几乎完全修复，对照组只有部分愈合。[结论]间充质干细胞不仅在体外具有良好的增殖能力，回植入体内后仍然具有良好的成骨能力，不需经过软骨过程就能直接形成新骨，显示了间充质干细胞作为骨组织工程种子细胞良好的应用前景。

     关键词：组织工程化骨；骨缺损；新生骨；间充质干细胞

    Study of the osteogenic ability of mesenchymal stem cells in vivo∥LI Zhanghua，PENG Hao,LIAO Wen，et alDepartment of Orthopaedics,Renmin Hospital,Wuhan University,Wuhan 430060

    Abstract：[Objective]To investigate the osteogenic ability in vivo of mesenchymal stem cells(MSCs)[Method]Tenfifteen ml bone marrow aspirates were harvested from the iliac crest of sheep and enriched for MSCs by density gradient centrifugation over a Percoll cushion(1073 g/ml)After cultured and proliferated,the tissueengineered bones were constructed with these cells and seeded onto porous 13tricalcium phosphate ceramics(13TCP)Then,the constructs were implanted into left metatarsus defect(21 mm in length)of 8 sheep as an experimental groupPorous βTCP composed with bone marrow was implanted into defects of same size and position in 8 sheep as a control groupSheep were sacrificed in the 6th,12th,and 24th week postoperatively,and the implanted samples were examined by radiograph,histology,and biomechanical analysis[Result]New bone tissue was observed either radiographically or histologically at the defect area of experimental group as early as the 6th week postoperatively;but was not observed in the control groupBecause of the new bone formed in a direct manner without progression through a cartilaginous process intermediately,osteoid tissue,woven bone and lamellar bone,occurred earlier in the experimental group than in the control group,in which the bone defects were repaired in a “creep substitution” mannerAt the 24th week,radiographs and biomechanical tests revealed an almost complete repair of the defect in experimental group,but only a partial repair in the control group[Conclusion]MSCs have good reproductive activity not only in vitro,but also in vivoThe new bone formed in a direct manner without progression through a cartilaginous process intermediately when MSCs were transplanted in vivoThe results demonstrated that MSCs was an excellent seed cells for bone tissue engineering ......

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