胰岛素_____胰岛素；聚乳酸；纳米粒；含量测定

紫外分光光度法测定胰岛素聚乳酸纳米粒中胰岛素的含量

http://www.100md.com 《广东药学院学报》 2001年第4期

胰岛素,,胰岛素；聚乳酸；纳米粒；含量测定,1药品与仪器,2实验方法与结果,3讨论,参考文献

     摘要建立一种简便易行测定胰岛素聚乳酸纳米粒中胰岛素含量的定量方法。以ψ(N，N-二甲基甲酰胺∶0.01 mol/L盐酸)=9∶1作溶剂溶解纳米粒沉淀后，紫外分光光度法测定吸光度，从而确定其中胰岛素的含量。检测波长276 nm，线性范围1.45～14.50 u/mL，平均回收率104.99%,测定结果稳定性与重现性良好。此方法操作简便、易于开展、结果可靠，应用于胰岛素聚乳酸纳米粒中胰岛素的含量测定可取得基本满意的效果。

    关键词胰岛素；聚乳酸；纳米粒；含量测定

    Determination of nsulin in insulin polylactide nanoparticles by ultraviolet spectrophotometry

    CHEN Jun1 ；YI Yimu2

    (1. Department of Pharmacy,Gulou Hospital,Nanjing 210008; 2. Department of Pharmaceutics,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030)

    Abstract A method for the determination of insulin(INS) in polylactide nanoparticles(PLANP) was established.The precipitate of INSPLANP was dissolved in N,N-dimethylformamide-0.01 mol/L HCl(9∶1). The content of INS in PLANP was determined by UV spectrophotometry with the wavelength of 276 nm. The standard cure was linear within 1.45~14.50 u/mL,and the average recovery was 104.99%. Both of the stability and reproducibility was good. This method was simple,reliable and satisfactory. ......

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