急性心肌缺血_

左旋精氨酸对急性心肌缺血大鼠L-及P-选择素表达的影响

http://www.100md.com 《广东药学院学报》 2001年第4期

急性心肌缺血,,急性心肌缺血；左旋精氨酸；选择素；大鼠,1材料和方法,2结果,3讨论,参考文献

     摘要目的：探讨NO的前体左旋精氨酸(Larg)对急性心肌缺血(AMI)大鼠心肌有无保护作用及其作用机制。方法：采用垂体后叶素(Pit)性急性心肌缺血模型，设立正常对照组、阳性对照组、及Larg治疗组，观察中性粒细胞(PMN)表面L-选择素及心肌微血管内皮细胞表面P-选择素的表达，并测定心肌梗死面积、PMN浸润数及血浆NO、MDA的浓度。结果：阳性对照组与正常对照组相比，其L-选择素及P-选择素表达上调，心肌PMN浸润数、血浆MDA的浓度明显升高，血浆NO的浓度降低。Larg治疗组上述指标改变的程度明显轻于阳性对照组(P<0.01)，心肌梗死范围小于阳性对照组。结论：外源性Larg对急性心肌缺血有防治意义，其作用的机制与NO抑制选择素的表达有关。

    关键词急性心肌缺血；左旋精氨酸；选择素；大鼠

    Effect of Larginine on expression of Land PSelectins in rats with acute myocardial ischemia

    LIU Ying CHEN Weiqiang

    (Department of Pathophysiology,Guangdong College of Pharmacy,Guangzhou,Guangdong 510224)

    Abstract Objective: To study the protective effect of NO precursorLarginine (Larg) on myocardium in rats with acute myocardial ischemia and its mechanism. Methods:A rat model of acute myocardial ischemia induced by pituitrin (Pit) was applied. 30 Wistar rats were divided into three groups : normal control group (injected with saline),positive control group (injected with 30 u/kg of Pit),and Larg treatment group (Pit injection followed by Larg injection). The expressions of Lselectin on neutrophils and Pselectin on myocardial microvascular endotheliocytes were determined. The myocardial infarct area,number of neutrophil infiltration and the levels of NO and MDA in plasma were determined. Results: In the positive control group (Pit group),the expressions of L and Pselectin,the number of neutrophil infiltration and the serum MDA increased,while the serum NO level decreased when compared with the normal control group. In the Larg treatment group,the changes of these indicators were significantly lesser than those in the Pit group (P<0.01),and the infarct area was smaller as well. Conclusion: The results suggested that Larg exerted significant cardioprotective effects in acute myocardial ischemia,and there was a positive correlation between the cardioprotection of Larg and the inhibition of NO on selectins expression. ......

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