口服乳剂__

去氢骆驼蓬碱口服乳剂体内外定量评价

http://www.100md.com 《广东药学院学报》 2002年第2期

口服乳剂,,口服乳剂；去氢骆驼蓬碱；定量评价；高效液相色谱法；体内分布,1仪器与试药,2实验方法与结果,3小结与讨论,参考文献

     摘要目的: 建立去氢骆驼蓬碱(harmine)口服乳剂的体内外含量测定方法，并研究该乳剂在小鼠体内的组织分布。方法: 采用高效液相色谱法(HPLC)测定口服乳剂中的harmine含量和各脏器组织中的药物浓度；色谱柱Shimpack CLCODS柱，流动相为ψ[乙腈∶甲醇∶磷酸盐缓冲液(pH6.8)]=30∶30∶40，流速1.0 mL/min，检测波长300 nm，柱温为室温。结果:方法学考察表明，标准曲线回归方程为A=151.33+87136x(r=0.9999)，线性范围0.0418～0.2508μg，平均回收率99.4%；小鼠体内试验显示，harmine乳剂组在胃、肺、肝、心等脏器中的药物分布均高于混悬液组，且胃内harmine水平大大高于其他脏器，并在较高浓度维持较长时间。结论: HPLC法简便可靠，可用于harmine乳剂的定量评价，该口服乳剂用于胃癌等消化道肿瘤有良好发展前景。

    关键词口服乳剂；去氢骆驼蓬碱；定量评价；高效液相色谱法；体内分布

    Quantitative evaluation of the oral harmine emulsion

    Abstract Objective: To establish a method for quantitative determination of harmine, and to study the tissue distribution of harmine emulsion (ig) in mice. Method: HPLC was used to determine harmine concentration in the oral emulsion or in tissues of mice. Stationary phase: Shimpack CLCODS column; mobile phase: acetonitribemethanolphosphate buffer (pH6.8) (30∶30∶40); mobile speed: 1.0 mL/min; detection wavelength: 300 nm; column temperature: 20℃. Results: Regression equation of standard curve was A=151.33+87136x (r=0.9999), and linear range was between 0.0418 and 0.2508μg; the average recovery was 99.4%. The results of the tissue distribution demonstrated that harmine levels in stomach, lungs, liver, brain and heart in the emulsion group were higher than those in the suspension group, with the highest conc entration in stomach. Conclusion: HPLC is simple and reliable, and can be used in the quantitative evaluation of the oral harmine emulsion. ......

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