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止咳枇杷冲剂中枇杷叶和桔梗的薄层色谱鉴别研究
http://www.100md.com 《广东药学院学报》 2002年第2期
枇杷叶,,薄层色谱;鉴别;枇杷叶;桔梗,1试剂、仪器、试药和药材,2实验方法,3讨论,参考文献
     摘 要 研究止咳枇杷冲剂中枇杷叶和桔梗的薄层色谱鉴别方法。分别取枇杷叶和桔梗药材、制剂及阴性制剂的醋酸乙酯提取液,在硅胶板上前者以ψ(氯仿∶甲醇∶水)=2∶2.25∶1.5展开,后者以ψ(正己烷∶氯仿∶甲醇)=5∶4∶0.3展开,磷钼酸乙醇溶液显色,在两者供试品色谱中与各自对照药材色谱相应位置上,分别有相同的蓝绿色特征斑点;阴性制剂色谱图中无此斑点,因此该方法可作为这两味中药的鉴别方法。

    关键词 薄层色谱;鉴别;枇杷叶;桔梗

    Studies on TLC identification of loquat leaf and platycodon root in the cough infusion of loquat leaf

    Abstract To study the TLC identification of the loquat leaf(LL) and platycodon root (PR) in the cough infusion of loquat leaf.The extracts of LL,PR and its preparation were subjected to TLC in different development systems.Chloroform methanol water(2∶2.25∶1.5) and nhexanechloroformmethanol (5∶4∶0.3) were used as development reagents for LL and PR respectively. Phosphomolybdic acidethanol solution was used for colouration, Results:After the development and coloration,bluegreen spots could be observed on positions corresponding to LL,PR and the preparation,while the negative control showed no spots. Conclusion:TLC method can be used in the identification of LL and PR in the cough infusion of loquat leaf. ......

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