洛美沙星_____洛美沙星；HPLC；胆汁;药物浓度

反相HPLC法测定胆汁中洛美沙星的浓度

http://www.100md.com 《广东药学院学报》 2002年第4期

洛美沙星,,洛美沙星；HPLC；胆汁;药物浓度,1实验材料,2实验方法,3实验结果,4讨论,参考文献

     摘要目的：建立用HPLC方法测定注射洛美沙星后不同时间的胆汁药物浓度。方法：胆汁样品离心后取上清液直接进样分析。色谱条件为C18柱(250 mm × 4.6 mm，5 μm)，流动相：ψ(0.05 mol/L柠檬酸液∶1 mol/L乙酸铵溶液∶乙腈)=77∶1∶14,流速：10 mL/min，检测波长：287 nm。结果：胆汁杂质对测定影响不大，线性方程相关系数大于0.999，平均回收率为85.28%。结论：方法专属性好，适用于临床胆汁中洛美沙星浓度测定。

    关键词洛美沙星；HPLC；胆汁;药物浓度

    Determination of lomefloxacine in bile by HPLC

    Abstract Objective: To establish a HPLC method for the determination of the drug concentration in bile after the injection of lomefloxacine. Methods: The bile sample was centrifuged and the supernatant was used in the determination. The mobile phase consisted of 0.05 mol/L citricacid-1 mol/L Ammonium acetate-acetonitrile(77∶1∶14).The YWC18(250 nm×46 mm,5 μm) column was used, with the velocity of flow at 1.0 mL/min and the detection wavelength of 287 nm. Results: The correlation coefficient of the linear equation was larger than 0.999,the average recovery was 85.28%. Conclusion: The method was simple, rapid and specific. It can be used for determination of Lomefloxacine in bile.

    Key words lomefloxacin; HPLC; bile;drug concentration

    洛美沙星(lomefloxacin ......

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