摘要 :目的 探讨HLA-DRB1等位基因多态性与慢性乙型肝炎之间的关系。 方法 采用聚合酶链反应、反向特异性序列寡核苷酸探针(SSO)基因分型技术结合Luminex液态芯片技术对81例慢性乙型肝炎患者和90例正常人的HLA-DRB1等位基因进行分型比较。 结果 HLA-DRB1等位基因0901型在慢性乙型肝炎的等位基因频率明显高于对照组(33.33%、15.56%)，两者相比差异有显著性(χ 2 =7.40，P<0.01，RR=2.71)，而HLA-DRB1等位基因0403型在慢性乙型肝炎的等位基因频率明显低于对照组(16.05%、35.56%)，两者相比差异有显著性(χ 2 =8.37，P<0.01，RR=0.37);其他型别的等位基因2组间没有明显差别。 结论 HLA-DRB1等位基因0901型与慢性乙型肝炎易感性密切相关，可能是慢性乙型肝炎的易感基因或连锁基因;HLA-DRB1等位基因0403型与慢性乙型肝炎抗性密切相关，可能是慢性乙型肝炎的抗性基因。宿主的HLA-DRB1是乙肝病毒感染转归的重要因素。

     关键词 :慢性乙型肝炎;HLA-DRB1基因;聚合酶链反应;反向特异性序列寡核苷酸探针;Luminex液态芯片技术;杂交

    A suudy on the association between chronic hepatitis B and HLA-DRB1

    FU Fei，CHEN Yong，LIU Xing-xiang

    (Department of Infectious Disease，Affiliated Hospital of Xuzhou Medical College，Xuzhou，Jiangsu221002，China)

    Abstract:Objective To investigate the association between chronic hepatitis B(CHB)and the polymorphism of HLA-DRB1.Methods The HLA-DRB1alleles in81patients with CHB and90normal control subjects were analyzed by using the polymerase chain reaction sequence specific oligonucleotide probes(PCR SSOP)technique and the Luminex liquid CMOS chip.Results The frequency of HLA-DRB10901allele in the CHB group was markedly higher than that in the normal control group(33.33%vs15.56% ......