抗癌胚抗原单链抗体的原核表达及对人胃癌的检测
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徐宏勇, 徐 立, 高建宏, 杨建军, 李开宗,
胃癌细胞;单链抗体;原核表达;癌胚抗原徐宏勇,徐立,高建宏,杨建军,李开宗,窦科峰.抗癌胚抗原单链抗体的原核表达及对人胃癌的检测. 世界华人消化杂志2006;14(18)1780-1784
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徐宏勇, 高建宏, 杨建军, 解放军第451医院肝胆普外科 陕西省西安市 710054
徐立, 第四军医大学西京医院全军消化病研究所 陕西省西安市 710032
李开宗, 窦科峰, 第四军医大学西京医院肝胆外科 陕西省西安市 710032徐宏勇, 2003年第四军医大学博士, 主治医师, 主要从事肝胆肿瘤疾病及免疫学治疗、肝移植等研究.
国家自然科学基金项目, No. 39900068
西安市科技攻关计划项目, No. GG05168
通讯作者: 徐宏勇, 710054, 陕西省西安市友谊东路269号, 解放军第451医院肝胆普外科. hongyongxu@medmail.com
电话: 029-84734128
收稿日期: 2006-04-16 接受日期: 2006-05-11
Prokaryotic expression of anti-carcinoembryonic single-chain variable fragment and its value in detection of gastric carcinoma cells
Hong-Yong Xu, Li Xu, Jian-Hong Gao, Jian-Jun Yang, Kai-Zong Li, Ke-Feng Dou
Hong-Yong Xu, Jian-Hong Gao, Jian-Jun Yang, Department of Hepatobiliary Surgery, the 451st Hospital of Chinese PLA, Xi’an 710054, Shaanxi Province, China
Li Xu, Institute of Digestive Disease, Xijing Hospital, the Fourth Medical University, Xi’an 710032, Shaanxi Province, China
Kai-Zong Li, Ke-Feng Dou, Department of Hepatobiliary Surgery, Xijing Hospital, the Fourth Medical University, Xi’an 710032, Shaanxi Province, China
Supported by National Natural Science Foundation of China, No. 39900068 and the Key Project from Science and Technology Foundation of Xi’an, Shaanxi Province, No. GG05168
Correspondence to: Dr. Hong-Yong Xu, Department of Hepatobiliary Surgery, the 451st Hospital of Chinese PLA, 269 Youyi East Road, Xi’an 710054, Shaanxi Province, China. hongyongxu@medmail.com.cn
Received: 2006-04-16Accepted: 2006-05-11
Abstract
AIM:To study the prokaryotic expression of the anti-carcinoembryonic antigen (CEA) single-chain fragment variable (scFv) antibody T84.66 and its specific affinity to gastric cancer cell lines and tissues.
METHODS:The cDNA of anti-CEA scFv antibody was inserted into pCANTAB5E to obtain phage vector T84.66-scFv-pCANTAB5E, and then the vector was transferred into E. coli HB2151 ......
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