Role of sodium selenite in regulating expressions of p38MAPK and MCPª²1 in rat mesangial cells

    WEI Qianª²Ping1, DENG Huaª²Cong1, ZHAO Jie2

    1Department of Endocrinology, First Affiliated Hospital, 2Institute of Ultrasound Engineering, Chongqing University of Medical Sciences, Chongqing 400016, China

    ¡¾Abstract¡¿ AIM: To observe the effect of sodium selenite on expressions of p38 mitogenª²activated protein kinase (p38MAPK) and monocyte chemoattractant proteinª²1 (MCPª²1) in rat mesanª²gial cell line HBZYª²1, and to study the mechanism of sodium selenite in preventing diabetic nephropathy. METHODS£º Cell line HBZYª²1 was incubated with high glucose, high insulin, H2O2 and advanced glycosylation end products (AGEs), respectively (control group); simultaneously, the cell line HBZYª²1 preª²treated with sodium selenite was also incubated with the above factors (experimental group). The expressions of p38MAPK and MCPª²1 were detected and compared in the 2 groups. RESULTS£º Four factors increased the expressions of p38MAPK and MCPª²1 indepently; sodium selenite inhibited the expressions of p38MAPK and MCPª²1 by the 4 factors distinctly. CONCLUSION£ºSodium selenite can suppress the expressions of p38MAPK and MCPª²1 in the cell line HBZYª²1, which suggests that sodium selenite may play a significant role in the prevention of diabetic nephropathy by the inhibition of the expressions of p38MAPK and MCPª²1 in the cell line HBZYª²1. ......