Differentially expressed proteins in renal tissues of rat models of chronic aristolochic acid nephropathy

    SUN Shiª²Ren1, CHEN Guangª²Lei1, NING Xiaoª²Xuan2, CHEN Wei1

    1Department of Nephrology, 2Department of Geratology, Xijing Hospital, Fourth Military Medical University, Xi¬ðan 710033, China

    ¡¾Abstract¡¿ AIM: To establish the 2ª²dimensional polyacrylamide gel electrophoresis (2ª²D PAGE) profiles from aristolochic acid nephropathy (AAN) rat renal tissues and normal renal tissues and to identify the differentially expressed proteins. METHODS: Establish chronic renal interstitial fibrosis model in rats by peritoneal injection with aristolochic acid (AA). The total proteins from AAN models and normal rat renal tissues were separated by 2ª²D PAGE. The differentially expressed proteins in 2Dª²PAGE maps were analyzed using image analysis software. RESULTS: The rat models of AAª²induced chronic renal interstitial fibrosis were established successfully. Clear, wellª²resolved and reproª²ducible 2ª²D PAGE patterns of AAN models and normal rat renal tissues were obtained and many differentially expressed proteins were found. CONCLUSION: The differentially expressed proteins of renal tissues can be induced by AA in Wistar rats. These proteins maybe mediate the toxicity of aristolochic acid to renal tissues. ......