Regulation of hCG on activity of transaldolase in K9 cell line

    YUAN Li, ZHANG Yongª²Ling, YUAN Li, ZHANG Yiª²Ming, MIAO Heng, DE Wei

    Department of Biochemisty and Molecular Biology, Nanjing Medical University, Nanjing 210029, China

    ¡¾Abstract¡¿ AIM: To study the regulation of human chorionic gonadotropin (hCG) on the activity of transaldolase (TAL) in K9 cell line. METHODS: In this research, K9 cells were cultivated in 3 groups, the 2 of which were incubated with hCG and cAMP respectively as the experimental groups and the other 1 without any factors as the control one. The activity of TAL was measured by Lachaise and the quantity of TAL proteins was detected by Western blot, the expression of TAL mRNA by Northern blot and the migration expression of the TAL protein by 2Dª²Western blot. RESULTS: (1) The expression of TAL mRNA was obviously enhanced by hCG, which was manifested by the fact that there was an increase of the TAL mRNA expression by 2 h (32.7¡À2.3) and a significant rising by 4 h (62.2¡À3.5) (P<0.05), while by 8h and 16h remarkable drops appeared (27.5¡À2.9, 18.1¡À1.1) respectively. In control group, the expression of TAL mRNA was (32.7¡À2.7), (25.2¡À1.9), (31.4¡À3.3) and (22.5¡À2.6) by 2, 4, 8 and 16 h respectively. (2) the expression level of TAL protein was not significantly different between hCG and control groups; (3) Both hCG and cAMP stimulated the activity of TAL, In the hCG group the activity of the TAL began to rise by 1 h of the experiment, and significantly increased by 2, 4, 8, 16 and 20 h during the process [P<0.05 vs control group (nkat/g); (417¡À33) vs (288¡À18) ......