摘要:目的 了解幽门螺杆菌(H.pylori，Hp)的抗原表型特征，并评价Hp抗原表型分型对临床诊治意义。 方法 以确诊感染Hp的132例胃肠疾病患者和20例无症状体检者作为研究对象。用免疫印迹法检测Hp抗体谱，分析相应的Hp细胞毒素相关蛋白(CagA)、空泡毒素(Vac A)、尿素酶(Urease)、鞭毛蛋白(Flagellin)及外膜蛋白(OMP)表达情况。 结果 Hp I型的感染率PU组(88.89%)、GC组(93.33%)明显高于健康体检组(35%)和CG组(51.85%)(P<0.005)。小分子量OMP的检出率GC组(100.00%)和CG组(60.49%)明显高于健康体检组(25%)和PU组(33.33%)(P<0.05)。 结论 Hp I型菌株比II型菌株致病力强更有可能增加胃癌发生风险，小分子量OMP可以作为胃癌及胃癌高发人群筛选的一种标志抗原;免疫印迹法是一种特异、敏感、可靠的确诊Hp感染的方法，值得推广应用。

    关键词:幽门螺杆菌;抗体谱;外膜蛋白;免疫印迹

    Clinical significance of antibody detection in diagnosis and treatment of patients with Helicobacter pylori infection in up-per gastrointestinal tract.

    YANG Lai-zhi，ZHANG Yin-hui，ZHANG Xiao-yan，et al.

    (Laboratory Department of Shenzhen Municipal Fourth Hospital，Shenzhen518033，Guangdong，P.R.China)

    Abstract:Objective To understand the characteristic of Helicobacter pylori(H.pylori，Hp)phenotypes and evaluate the clin-ical significance of phenotype differentiation. Methods 132patients with gastroduodenal diseases and20volunteers without symp-toms were observed.132patients were diagnosed as chronic gastritis(CG)(n=81) ......