摘要:目的 探讨随机扩增多态性DNA(Randomly amplified polymorphic DNA，RAPD)技术在分枝杆菌分型诊断中优化条件及其应用价值。 方法 优化RAPD技术扩增分枝杆菌DNA的条件，并分别扩增深圳市临床病人分离株DNA，对比RAPD电泳条带特征。 结果 以条带稳定、丰富、清晰为标准，确定引物1RAPD最佳反应条件为:50μl反应体系中，含100ng模板DNA，2.5mmol/L MgCl 2 ，2U DNA聚合酶，引物0.5μmol/L，dATP、dGTP、dCTP和dTTP各250μmol/L，反应40个循环，每个循环为:94℃1min，36℃1min，72℃2min。所扩增结核杆菌临床分离株的DNA条带丰富、清晰，各条带差异较明显。 结论 RAPD技术能较好地鉴别MTB和NTM。

     关键词:分枝杆菌;非典型性;结核杆菌;RAPD;分型

    The optimized condition for differentiation of Mycobactreium strains by using RAPD and its appliation in strain typing and diagnosis.

    X Li，YANG Ying-zhou，TAN Wei-guo，et al.

    (Shenzhen Municipal Chronic Disease Hospital，Shenzhen518020，Guangdong，P.R.China)

    Abstract:Objective To explore the optimized condition for type differentiation of Mycobacteria strains and its value in applifi-cation by means of randomly amplified polymorphic DNA(RAPD)analysis. Methods RAPD condition for amplifying Mycobacteria DNA of clinically separated Mycobacteril strains from patients in Shenzhen was optimized ......