摘要:目的 检测疟原虫HGXPRT蛋白刺激淋巴细胞的增殖效果。 方法 以HGXPRT蛋白免疫小鼠后制备淋巴细胞，与相应刺激物共同培养后采用CCK-8法检测淋巴细胞的增殖情况。 结果 不同浓度的HGXPRT对淋巴细胞的刺激效果不同，以5μg/ml时刺激增殖效果最好。 结论 HGXPRT对淋巴细胞具有增殖作用。

    关键词:HGXPRT蛋白;淋巴细胞增殖;CCK-8;疟原虫

    Detection of lymphocyte proliferation stimulated by HGXPRT protein of Plasmodium falciparum.

    WANG Bing，ZHANG Dong-mei，PAN Wei-qing.

    (Department of Etiologic Biology，Second MilitaryMedical University，Shanghai200433，P.R.Chi-na)

    Abstract:Objective To determine the effect of proliferation of lymphocytes stimulated by Plasmodium falciparum HGXPRT protein. Methods Lymphocytes from BALB/c mice immunized with Plasmodium falciparumHGXPRT protein were prepared and the proliferation of lymphocytes was determined by CCK-8after jointly cultured with corresponding stimulators. Results Effects of stimulation depended on the concentration of Plasmodium falciparumHGXPRT and the concentration of5μg/ml was the most effica-cious for the proliferation of lymphocytes. Conclusion Plasmodium falciparumHGXPRT can stimulate the proliferation of lym-phocytes. ......