乙型肝炎血清标志物与HBV DNA的相关性分析
乙型肝炎;FQ-PCR;HBV-DNA;HBV-M,,乙型肝炎;FQ-PCR;HBV-DNA;HBV-M,乙型肝炎血清标志物与HBVDNA的相关性分析,1材料和方法,2结果,3讨论,参考文献:
摘要:目的 探讨乙型肝炎患者血清标志物(HBV-M)与HBV-DNA的关系。 方法 采用酶联免疫吸附试验(ELISA)和荧光定量-PCR(FQ-PCR)法对1433例乙肝患者进行HBV-M以及HBV-DNA定量检测。 结果 在HBsAg(+)HBeAg(+)HBcAb(+)血清中HBV-DNA阳性率和含量最高，血清中HBeAg与HBV-DNA含量密切相关，但部分HBeAg阴性或HBeAb阳性患者也有较高的HBV-DNA阳性率及含量。 结论 单凭血清免疫标志物模式难以准确判断HBV的复制程度及传染性的强弱，定量检测HBV-DNA能真实反映HBV的复制情况，对诊断、治疗乙肝患者及疗效观察具有较大的指导意义。关键词:乙型肝炎;FQ-PCR;HBV-DNA;HBV-M
The relationship between serum markers in hepatitis B patients and HBV DNA and clinical significance.
LI Zhi-fang，WU Xiao-man.
(Laboratory Department of Second Affiliated Hospital of Guangdong Medical College，Guangzhou510260，Guang-dong，P.R.China)
Abstract:Objective To analyze the relationship between serum HBV-DNA levels and the patterns of serological markers. Methods The levels of HBV markers and HBV-DNA in the serum of1433hepatitis B patients were quantitatively determined by ELISA and FQ-PCR. Results There was a close relationship between serum HBV-DNA and HBeAg.The highest HBV-DNA levels were presented in sera positive both for HBsAg and HBeAg ......
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