摘要:目的 探讨双试剂双缩脲法在全自动生化仪中直接测定脂肪乳浊标本血清总蛋白。 方法 将单试剂双缩脲法改成双试剂，在全自动生化仪上采用二点终点法直接测定。 结果 双试剂双缩脲法测定脂浊血清标本的总蛋白为(74.27±5.65)mmol/L与手工标化法测定的(74.46±5.77)mmol/L差异无显著性(P>0.05)。 结论 双试剂双缩脲法能有效地消除脂肪的干扰，无需另进行手工除脂测定，大大地提高工作效率。

    关键词:双试剂;双缩脲法;血清总蛋白;脂浊标本

    Determination of total serum from fat blood specimens by using biurea and double reagents.

    XIE Zhen-ji，LUO Qiao-zhuang，ZHOU Shao-qing.

    (Fanyu District Dashi Hospital of Guangzhou City，Guangzhou511430，Guangdong，P.R.China)

    Abstract:Objective To discuss the biurea method for determination of total protein in serum of fat emulsion specimens with double reagents on the automated biochemical analyzer. Methods The single reagent biurea reaction was altered to the double reagent and direct determination was performed by using the two-end-point method on the automated biochemical analyzer. Re-sults There were no marked differences between the double reagent reaction and biurea manual standard reduction. Conclusion The interference of the fat can be effectively eliminated by double reagent biurea reaction without need to carry on manual determina-tion of fat ......