[摘要]目的：探讨RhoA在洛伐他汀抑制HepG2细胞增殖过程中所起的作用。方法：用基因芯片、RT_PCR和Western blot检测洛伐他汀作用前、后HepG2细胞中RhoA的表达。结果：加入洛伐他汀后，mRNA(RhoA)和RhoA蛋白水平均上调；加入香叶基香叶基焦磷酸或法呢基焦磷酸后，RhoA蛋白水平上调被不同程度逆转。结论：减少RhoA香叶酯化，抑制RhoA的细胞膜锚着可能是洛伐他汀抑制HepG2增殖的机制之一。

    [关键词]羟甲基戊二酰辅酶A还原酶抑制剂；HepG2细胞；增殖；RhoA

    Role of RhoA in Lovastatin_inhibited Proliferation of HepG2 Cells

    WU Bao_an, LIANG Li_jian, LI Wei, SONG Xiang_ru

    (1Department of General Surgery, The First Affiliated Hospital, Shantou University Medical College, Shantou 515041，China;

    2Department of Hepatobiliary Surgery, The First Affiliated Hospital, Sun Yat_sen University, Guangzhou 510080，China)

    [Abstract]Objective: To study the role of RhoA in lovastatin_inhibited proliferation of HepG2 cells. Methods: The cDNA microarray assay, RT_PCR and Western blot were carried to analyze the mRNA and protein expression of RhoA, respectively. Results: The mRNA and protein expression levels of RhoA were up_regulated in lovastatin_inhibited HepG2 cells. This response could be reversed by addition of geranylgeranyl pyrophosphate or farnesyl pyrophosphate in different degrees. Conclusion: RhoA isoprenylation inhibition and its delocalization from the cell membrane may be one of the mechanisms of lovastatin_inhibited anti_proliferation effect in HepG2 cells. ......