(1. Institute of Medicinal Plant Development, Peking Union Medical College, Chinese Academy of Medical Sciences, Malianwa North Road 151#, Beijing 100094, China; 2.Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Xian Nong Tan Street 1#, Beijing 100050, China; 3. Department of Chemical Engineering, Tsinghua University, Beijing 100084, China)

    Abstract:ObjectiveTo analyze the components in callus and cell suspension cultures of Ligusticum chuanxiong Hort. by gas chromatography-mass spectrometry (GC-MS). Methods Initiate the callus and cell suspension cultures of Ligusticum chuanxiong Hort.. Collect the callus and three sequent generations cell suspension cultures, and the components in callus and cell suspension cultures were analyzed by gas chromatography-mass spectrometry. ResultsThe callus and cell suspension cultures of Ligusticum chuanxiong Hort. contained similar constituenents, but the components in callus and cell suspension cultures were quite different from those of the plant, and the main bioactive constituentes of the plant were not found in callus and cell suspension cultures. ConclusionThe characteristics of callus and cell suspension cultures were different from those of the plant. ......