【摘要】 目的 探讨中国北方汉族人动脉硬化性脑梗死(atherothrombotic cerebral infarction,ABI)易感的分子机制和确立一种较为实用的人类主要组织相容性复合体(major histocompatibility complex,MHC)-DRB1*等位基因的检测方法。方法 采用聚合酶链式反应和顺序特异性引物(polymerase chain reaction-sequence specific primer,PCR-SSP)基因分析方法，对36例ABI患者及32例无血缘关系的健康人的MHC-DRB1*各等位基因进行了检测分析，并将该方法与其他3种检测MHC基因的方法进行对比。结果 结果表明，MHC-DRB1*0301基因与ABI呈正相关(RR=5.46，P<0.05)，其他MHC-DRB1*各等位基因均无统计学差异。结论 本项研究结果提示MHC-DRB1*0301基因可能是北方汉族人ABI的致病易感基因之一，为揭示ABI的发病机制中免疫遗传学机制所起的作用提供了重要信息和依据。尽管目前MHC-DR基因分型方法已有多种，但本文所采用的方法具有快速、简便、准确和可靠等优点，值得推广应用。

    【关键词】 MHC-DRB1*；脑梗死；易感基因

    MHC-DRB1*gene polymorphism in patients with atherothrombotic cerebral infarction

    QUAN Zan-rong，CHEN Li-jie.Department of Neurology,The Second Affiliated Hospital,Harbin Medical University,Harbin 150036,China

    【Abstract】 Objective To observe the correlativity between MHC-DRB1*gene polymorphism and atherothrombotic cerebral infarction(ABI).Methods MHC-DRB1*gene polymorphism in 36 patients with ABI and 32 normal control subjects were analyzed by using the polymerase chain reaction/sequence specific primer(PCR/SSP)technique.Results The frequency of MHC-DRB1*0301 allele was significantly higher in patients than that in the controls,RR of ABI patients was 5.46,P<0.05.Conclusion These findings further verify that immunogenetics play a predominant pathogenetics role in a subset of ABI patients.Individuals carrying MHC-DRB1 *0301 alleles may be susceptibility gene to ABI.This method is more specific,sensitive,simple,rapid and accurate,and could be applied to the routine clinical detection. ......