关键词 红斑狼疮，系统性；白细胞介素；干扰素

    摘要 目的 探讨系统性红斑狼疮(SLE)患者血清IL-13、IL-12、IL-2和IFN-γ水平变化及意义。方法 采用双抗体夹心ELISA法检测58例SLE患者(活动期16例，稳定期42例)及30例健康对照者血清IL-13、IL-12、IL-2和IFN-γ的含量。结果 SLE活动组、稳定组血清IL-13、IFN-γ水平与对照组比较明显增高，差异有统计学意义(P<0.01),活动组IL-13又较稳定组增高，差异亦有统计学意义(P<0.05),IFN-γ水平略高于稳定组,但差异无统计学意义；IL-12、IL-2与对照组比较，稳定组略高，活动组略低，差异无统计学意义，但疾病活动组与稳定组比较则明显降低，差异有统计学意义(P<0.05)。SLE活动期IL-2与IL-13呈负相关(r=-0.52,P<0.05)。结论 SLE患者存在细胞因子紊乱，IL-13、IL-12、IL-2及IFN-γ可能参与SLE的发病、发展，且IL-13、IL-12、IL-2水平与SLE疾病活动有关。

    SERUM LEVELS OF IL-13,IL-12,IL-2 AND IFN-γ IN PATIENTS WITH SYSTEMIC LUPUS ERYTHEMATOSUS

    Zou Jimin, Yuan Baojun, An Xin, et al.

    Department of Clinical Laboratory, The Attached Kailuan Hospital of North Coal Medical College, Tangshan 063000,China

    Key words lupus erythematosus; systemic; interleukin； IFN-γ

    Abstract Objective To investigate the changes of serum IL-13, IL-12, IL-2, and IFN-γ levels in patients with systemic lupus erythematosus. Methods The levels of serum IL-13, IL-12, IL-2, and IFN-γ were determined by ELISA,58 patients with SLE were divided as active group(n=16) and stable group(n=42). As control, 30 healthy people were also recruited. Results The levels of IL-13 and IFN-γ in patients with SLE were significantly higher than those in controls(P<0.01),serum IL-13 level in the active group was higher than that in the stable group(P<0.05).Compared with control, there was no significant difference in the contents of serum IL-12 and IL-2 in two SLE groups(P>0.05),although the levels in active SLE group were slightly lower and those in the stable SLE group were slightly higher; serum IL-12 and IL-2 levels in the active group were significantly lower compared with those in the stable group(P<0.05).There was negative correlation between IL-13 and IL-12 in active group(r=-0.52,P<0.05). Conclusion Cytokine imbalance exists in patients with SLE, serum levels of IL-13, IL-12, IL-2, and IFN-γ might participate in immunopathological mechanism of SLE and IL-13,IL-12,IL-2 are associated with SLE activity. ......