IL6；逆转录；pcDNA3.1

大鼠IL6基因真核表达质粒的构建新疆维吾尔自治区教育厅高校重点资助项目(XJEDU2004I26)

http://www.100md.com 《新疆医科大学学报》 2006年第6期

IL6；逆转录；pcDNA3.1,CTGFPTOPO,,IL6；逆转录；pcDNA3.1,CTGFPTOPO,大鼠IL6基因真核表达质粒的构建新疆维吾尔自治区教育厅高校重点资助项目

     摘要: 目的：为应用RNAi技术调节白细胞介素6(interleukin6，IL6)基因表达，构建大鼠IL6基因真核表达质粒。方法：从大鼠结肠中提取总RNA，经逆转录(reverse transcription)得到目的基因的cDNA，经PCR得到目的基因扩增片段，将其连接入pcDNA3.1/CTGFPTOPO载体中，对重组体进行鉴定。结果：成功构建了目的基因的重组体，测序报告显示核苷酸序列无误。结论：大鼠真核表达质粒pcDNA3.1/CTGFPTOPO/IL6的成功构建，为应用RNAi技术调节IL6基因表达提供了实验基础，为研究IL6与溃疡性结肠炎的关系提供实验平台。

     关键词：IL6；逆转录；pcDNA3.1/CTGFPTOPO

    The construction of rat interleukin6 gene eukaryotic expression vector

    HAN Mingguo, ZHAO Xuexin, Hamulati·Wupuer, et al

    (Department of Biochemistry, Preclinical Medicine College, Xinjiang Medical University，Urumqi 830054, China)

    Abstract: Objective: We construct an eukaryotic expression recombinant containing the rat IL6 gene to regulate IL6 gene expression with RNAi technique. Methods: The rat IL6 cDNA was reverse transcripted from total RNA which was extracted from rat's colon, amplified by PCR, then it was inserted into the vector pcDNA3.1/CTGFPTOPO. Result: Sequencing results showed that an eukaryotic expression vector ......

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